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Mouse TNF RII/TNFRSF1B Antibody

Catalog #: AF-426-PB
12 Citations Datasheet / COA / SDS
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AF-426-PB
AF-426-SP
Detection of Mouse TNF RII/TNFRSF1B by Western Blot.
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Citations (12)
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Mouse TNF RII/TNFRSF1B Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TNF RII in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TNF RII is observed and less than 1% cross-reactivity with recombinant mouse (rm) CD27, rmGITR, rhDR6, rmCD30, rmCD40, rmEDAR, rmFas, rmOPG, rmRANK, and rmTNF RI is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse TNF RII/TNFRSF1B
Val23-Gly258
Accession # P25119
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse TNF RII/TNFRSF1B Monoclonal Antibody (Catalog # MAB4263).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse sTNF RII/TNFRSF1B DuoSet ELISA Kit (Catalog # DY426) for convenient development of a sandwich ELISA or the Mouse/Rat TNF RII/TNFRSF1B Quantikine ELISA Kit (Catalog # MRT20) for a complete optimized ELISA.

 
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
1.7-15 µg/mL
Immersion fixed L‑929 mouse fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse TNF RII/TNFRSF1B antibody by Western Blot. View Larger

Detection of Mouse TNF RII/TNFRSF1B by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RII/TNFRSF1B at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry View Larger

Detection of TNF RII/TNFRSF1B in Raw264.7 Mouse Cell Line by Flow Cytometry. Raw264.7 mouse macrophage cell line, resting (open histogram) or treated with 1 μg/mL LPS overnight (filled histogram), was stained with Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Staining was performed using our Staining Membrane-associated Proteins protocol.

Flow Cytometry Detection of TNF RII/TNFRSF1B antibody in L-929 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TNF RII/TNFRSF1B in L‑929 Mouse Cell Line by Flow Cytometry. L-929 mouse fibroblast cell line was stained with Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Immunocytochemistry View Larger

TNF RII/TNFRSF1B in L‑929 Mouse Cell Line. TNF RII/TNFRSF1B was detected in immersion fixed L‑929 mouse fibroblast cell line using Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RII/TNFRSF1B

Two types of soluble TNF receptors have been identified in human serum and urine which can neutralize the biological activities of TNF-alpha and TNF-beta. These binding proteins represent truncated forms of the two types of high-affinity cell surface receptors for TNF (TNFR-p60 Type B and TNFR-p80 Type A). Soluble TNF RII corresponds to TNFR-p80 Type A. In the new TNF superfamily nomenclature, TNF RII is referred to as TNFRSF1B. These apparent soluble forms of the receptors appear to arise as a result of shedding of the extracellular domains of the membrane-bound receptors. Normal concentrations as high as 4 ng/mL are found in the serum of healthy individuals, and even higher levels may be found in some pathological conditions. Although the physiological role of these proteins is not known, it has been speculated that shedding of the soluble receptors in response to TNF release could serve as a mechanism to scavenge the TNF not immediately bound and thus localize the inflammatory response. It is also possible that the pool of TNF bound to soluble receptors could represent a reservoir for the controlled release of TNF.

Long Name
Tumor Necrosis Factor Receptor II
Entrez Gene IDs
7133 (Human); 21938 (Mouse); 156767 (Rat); 102144224 (Cynomolgus Monkey)
Alternate Names
CD120b antigen; CD120b; Etanercept; p75 TNF receptor; p75TBPII; p75TNFR; soluble TNFR1B variant 1; TNF RII; TNFBRp80 TNF-alpha receptor; TNF-R2; TNFR2TNFR1B; TNF-R75; TNFR80; TNFRII; TNF-RII; TNF-R-II; TNFR-II; TNFRSF1B; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2; Tumor necrosis factor receptor 2; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B; Tumor necrosis factor receptor type II

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Citations for Mouse TNF RII/TNFRSF1B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Microglial TNFR2 signaling regulates the inflammatory response after CNS injury in a sex-specific fashion
    Authors: Raffaele, S;Thougaard, E;Laursen, CCH;Gao, H;Andersen, KM;Nielsen, PV;Ortí-Casañ, N;Blichfeldt-Eckhardt, M;Koch, S;Deb-Chatterji, M;Magnus, T;Stubbe, J;Madsen, K;Meyer, M;Degn, M;Eisel, ULM;Wlodarczyk, A;Fumagalli, M;Clausen, BH;Brambilla, R;Lambertsen, KL;
    Brain, behavior, and immunity
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. The Inflammatory Response after Moderate Contusion Spinal Cord Injury: A Time Study
    Authors: MC Lund, DG Ellman, M Nissen, PS Nielsen, PV Nielsen, C Jørgensen, DC Andersen, H Gao, R Brambilla, M Degn, BH Clausen, KL Lambertsen
    Biology, 2022-06-20;11(6):.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC
  3. Apoptosis signal-regulating kinase 1 activation by Nox1-derived oxidants is required for TNF alpha receptor endocytosis
    Authors: Hyehun Choi, Ryan J. Stark, Benjamin S. Raja, Anna Dikalova, Fred S. Lamb
    American Journal of Physiology-Heart and Circulatory Physiology
  4. Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2
    Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann
    Sci Rep, 2017-07-26;7(1):6607.
    Species: Mouse
    Sample Types: Whole Cells
  5. Endogenous TNF? orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation
    Authors: S Arokiasamy, C Zakian, J Dilliway, W Wang, S Nourshargh, MB Voisin
    Sci Rep, 2017-03-13;7(0):44189.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Phenotypic screening reveals TNFR2 as a promising target for cancer immunotherapy
    Oncotarget, 2016-10-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Tumor necrosis factor (TNF)-receptor 1 and 2 mediate homeostatic synaptic plasticity of denervated mouse dentate granule cells
    Authors: Denise Becker, Thomas Deller, Andreas Vlachos
    Scientific Reports
  8. Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients.
    Authors: Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S
    J Immunol, 2014-07-16;193(4):2024-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Myosin light chain kinase expression induced via tumor necrosis factor receptor 2 signaling in the epithelial cells regulates the development of colitis-associated carcinogenesis.
    Authors: Suzuki M, Nagaishi T, Yamazaki M, Onizawa M, Watabe T, Sakamaki Y, Ichinose S, Totsuka M, Oshima S, Okamoto R, Shimonaka M, Yagita H, Nakamura T, Watanabe M
    PLoS ONE, 2014-02-10;9(2):e88369.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
    Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
    J. Exp. Med., 2012-05-21;209(6):1219-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Injury-Induced Neurogenesis: Consideration of Resident Microglia as Supportive of Neural Progenitor Cells
    Authors: Christopher A. McPherson, Andrew D. Kraft, G. Jean Harry
    Neurotoxicity Research
  12. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
    Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
    J. Clin. Invest., 2009-09-14;119(10):2954-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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