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Mouse TORC1 Antibody

Catalog #: MAB5978 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5978
MAB5978-SP
Product Details
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Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TORC1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TORC1 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 505531
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse TORC1
Gln384-Gln471
Accession # Q68ED7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of mouse brain (hippocampus)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TORC1

TORC1, also known as CREB-regulated transcription coactivator 1 (crtc1), is a 67 kDa member of the TORC family. Mouse TORC1 is 630 amino acids (aa) in length. It contains multiple potential sites for phosphorylation and a serine-rich region from aa 295‑365. Mouse TORC1 is 97% and 90% aa identical to rat and human TORC1, respectively. TORC1 is highly expressed in specific regions of the brain including the cortex, hippocampus, and striatum. It functions as a transcription coactivator for CREB1, which activates transcription through both consensus and variant cAMP response element (CRE) sites.

Long Name
Transducer of Regulated CREB protein 1
Entrez Gene IDs
23373 (Human); 382056 (Mouse)
Alternate Names
CREB regulated transcription coactivator 1; CRTC1; FLJ14027WAMTP1; KIAA0616Transducer of regulated cAMP response element-binding protein 1; MECT1; MECT1CREB-regulated transcription coactivator 1; mucoepidermoid carcinoma translocated 1; Mucoepidermoid carcinoma translocated protein 1; TORC1; TORC1TORC-1; Transducer of CREB protein 1; transducer of regulated cAMP response element-binding protein (CREB) 1; WAMTP1

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Mouse TORC1 Antibody
By Anonymous on 07/19/2019
Application: WB Sample Tested: Adipose tissue Species: Mouse
Western Blot Mouse TORC1 Antibody MAB5978