Home / TRANCE/TNFSF11/RANK L / Mouse TRANCE/TNFSF11/RANK L Antibody

Mouse TRANCE/TNFSF11/RANK L Antibody

Catalog #: MAB462
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB462
MAB462-SP
Product Details
Citations (1)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TRANCE/TNFSF11/RANK L Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TRANCE in direct ELISAs and Western blots. In direct ELISAs, shows 100% cross-reactivity with recombinant human (rh) TRANCE and no cross-reactivity with rhAPRIL, rhBAFF, rhCD27 Ligand, recombinant mouse (rm) CD27 Ligand, rhCD30 Ligand, rmCD30 Ligand, rhCD40 Ligand, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, rhTNF-alpha, rhTRAK, rmTSG, recombinant cotton rat TNF‑ alpha, rmTNF-alpha, recombinant porcine TNF-alpha, recombinant rat TNF-alpha, and rhVEGI.
Source
Monoclonal Rat IgG2A Clone # 88227
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TRANCE
Arg72-Asp316
Accession # O35235
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRANCE/TNFSF11/RANK L

TRANCE, also known as RANK Ligand, is a type II transmembrane protein belonging to the tumor necrosis factor superfamily and has been designated TNFSF11.

Long Name
TNF-related Activation-induced Cytokine
Entrez Gene IDs
8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)
Alternate Names
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11

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Citation for Mouse TRANCE/TNFSF11/RANK L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Bacterial lipopolysaccharide induces osteoclast formation in RAW 264.7 macrophage cells.
    Authors: Islam S, Hassan F, Tumurkhuu G, Dagvadorj J, Koide N, Naiki Y, Mori I, Yoshida T, Yokochi T
    Biochem. Biophys. Res. Commun., 2007-06-12;360(2):346-51.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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