Home / TRANCE/TNFSF11/RANK L / Mouse TRANCE/TNFSF11/RANK L Antibody

Mouse TRANCE/TNFSF11/RANK L Antibody

Catalog #: AF462
34 Citations 2 Reviews Datasheet / COA / SDS
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AF462
AF462-SP
Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody.
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Citations (34)
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Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TRANCE/TNFSF11/RANK L Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TRANCE/TNFSF11/RANK L in ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TRANCE/TNFSF11/RANK L
Arg72-Asp316
Accession # O35235
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR)
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse thymus

Mouse TRANCE/TNFSF11/RANK L Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse TRANCE/TNFSF11/RANK L Biotinylated Antibody (Catalog # BAF462)

Standard: Recombinant Mouse TRANCE/RANK L/TNFSF11 Protein (Catalog # 462-TR)

Neutralization
Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in mouse splenocytes. Nakagawa, N. et al. (1998) Biochim. Biophys. Res. Commun. 253:395. The Neutralization Dose (ND50) is typically 0.02-0.06 µg/mL in the presence of 30 ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody. View Larger

Osteoclast-like Cell Formation Induced by TRANCE/ TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody. In the presence of Recombinant Mouse M-CSF (20 ng/mL, 416-ML), Recombinant Mouse TRANCE/TNFSF11/RANK L (462-TR) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Mouse TRANCE/TNFSF11/ RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRANCE/TNFSF11/RANK L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF462). The ND50 is typically 0.02-0.06 µg/mL.

Immunohistochemistry-Paraffin Detection of Mouse TRANCE/TNFSF11/RANK L by Immunohistochemistry-Paraffin View Larger

Detection of Mouse TRANCE/TNFSF11/RANK L by Immunohistochemistry-Paraffin MMP2, MMP9, and RANKL expression in AAAs.A. Immunohistochemical staining for indicated proteins of serial sections of aortas one week after CaCl2 treatment. Elastic van Gieson staining is also shown. SM alpha -actin and F4/80 were stained to locate SMCs and macrophages, respectively. Shown are representative images of 4 or more samples in each group. Scale bars, 50 µm. B. Relative positive staining area of MMP2, MMP9, and RANKL in sections from control diet and EPA diet groups. n = 4–5. *P<0.05. Image collected and cropped by CiteAb from the following open publication (https://dx.plos.org/10.1371/journal.pone.0096286), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TRANCE/TNFSF11/RANK L

TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

References
  1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
  2. Anderson, D.M. et al. (1997) Nature 390:175.
  3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
  4. Kong, Y-Y. et al. (1999) Nature 397:315.
Long Name
TNF-related Activation-induced Cytokine
Entrez Gene IDs
8600 (Human); 21943 (Mouse); 117516 (Rat); 102137946 (Cynomolgus Monkey)
Alternate Names
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11

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Citations for Mouse TRANCE/TNFSF11/RANK L Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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  1. Aberrant Activation of the RANK Signaling Receptor Induces Murine Salivary Gland Tumors
    Authors: Maria M. Szwarc, Ramakrishna Kommagani, Allison P. Jacob, William C. Dougall, Michael M. Ittmann, John P. Lydon
    PLOS ONE
  2. Single-Cell RNA Sequencing of Lymph Node Stromal Cells Reveals Niche-Associated Heterogeneity
    Authors: Lauren B. Rodda, Erick Lu, Mariko L. Bennett, Caroline L. Sokol, Xiaoming Wang, Sanjiv A. Luther et al.
    Immunity
  3. The transcriptional co-factor RIP140 regulates mammary gland development by promoting the generation of key mitogenic signals
    Authors: Jaya Nautiyal, Jennifer H. Steel, Meritxell Rosell Mane, Olayiwola Oduwole, Ariel Poliandri, Xanthippi Alexi et al.
    Development
  4. Propionibacterium freudenreichii MJ2-derived extracellular vesicles inhibit RANKL-induced osteoclastogenesis and improve collagen-induced rheumatoid arthritis
    Authors: Woo, HE;Cho, JY;Lim, YH;
    Scientific reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  5. Mechanosensitive hormone signaling promotes mammary progenitor expansion and breast cancer risk
    Authors: Northey, JJ;Hayward, MK;Yui, Y;Stashko, C;Kai, F;Mouw, JK;Thakar, D;Lakins, JN;Ironside, AJ;Samson, S;Mukhtar, RA;Hwang, ES;Weaver, VM;
    Cell stem cell
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  6. Differential osteoblastic activity in primary metaphyseal trabecular and secondary trabeculae of c-fos deficient mice
    Authors: Yamamoto, T;Abe, M;Hongo, H;Maruoka, H;Yoshino, H;Haraguchi-Kitakamae, M;Udagawa, N;Li, M;Amizuka, N;Hasegawa, T;
    Journal of oral biosciences
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. RANKL up-regulated by progesterone aggravates lipopolysaccharide-induced acute lung injury during pregnancy
    Authors: ZZ Lai, WJ Zhou, JW Shi, YH Meng, JN Wu, JF Ye, T Peng, CE Xu, MQ Li
    Journal of reproductive immunology, 2022-12-16;155(0):103788.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Targeting hepatic oxidative stress rescues bone loss in liver fibrosis
    Authors: S Sonoda, S Murata, H Yamaza, R Yuniartha, J Fujiyoshi, K Yoshimaru, T Matsuura, Y Oda, S Ohga, T Tajiri, T Taguchi, T Yamaza
    Molecular Metabolism, 2022-09-13;66(0):101599.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Histopathologic and transcriptomic phenotypes of a conditional RANKL transgenic mouse thymus
    Authors: MM Szwarc, L Hai, VK Maurya, K Rajapakshe, D Perera, MM Ittmann, Q Mo, Y Lin, ML Bettini, C Coarfa, JP Lydon
    Cytokine, 2022-09-11;160(0):156022.
    Species: Transgenic Mouse
    Sample Types: Protein, Whole Tissue
    Applications: IHC, Western Blot
  10. Activation function 1 of progesterone receptor is required for mammary development and regulation of RANKL during pregnancy
    Authors: SH Lee, YHY Yap, CL Lim, ARE Woo, VCL Lin
    Scientific Reports, 2022-07-19;12(1):12286.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Mechanisms involved in suppression of osteoclast supportive activity by transforming growth factor-beta1 via the ubiquitin-proteasome system
    Authors: M Inoue, Y Nagai-Yosh, R Yamasaki, T Kawamoto, T Nishihara, W Ariyoshi
    PLoS ONE, 2022-02-23;17(2):e0262612.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Oxytocin receptor induces mammary tumorigenesis through prolactin/p-STAT5 pathway
    Authors: D Li, M San, J Zhang, A Yang, W Xie, Y Chen, X Lu, Y Zhang, M Zhao, X Feng, Y Zheng
    Cell Death & Disease, 2021-06-07;12(6):588.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Osteocyte RANKL is required for cortical bone loss with age and is induced by senescence
    Authors: HN Kim, J Xiong, RS MacLeod, S Iyer, Y Fujiwara, KM Cawley, L Han, Y He, JD Thostenson, E Ferreira, RL Jilka, D Zhou, M Almeida, CA O'Brien
    JCI Insight, 2020-10-02;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  14. Antiresorptive activity of osteoprotegerin requires an intact heparan sulfate-binding site
    Authors: M Li, D Xu
    Proc. Natl. Acad. Sci. U.S.A., 2020-07-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC, Proximity Ligation Assay
  15. The RNA binding protein CPEB2 regulates hormone sensing in mammary gland development and luminal breast cancer
    Authors: R Pascual, J Martín, F Salvador, O Reina, V Chanes, A Millanes-R, C Suñer, G Fernández-, A Bartomeu, YS Huang, RR Gomis, R Méndez
    Sci Adv, 2020-05-15;6(20):eaax3868.
    Species: Human
    Sample Types: Tissue
    Applications: IHC
  16. Short-term RANKL exposure initiates a neoplastic transcriptional program in the basal epithelium of the murine salivary gland
    Authors: L Hai, MM Szwarc, DM Lonard, K Rajapakshe, D Perera, C Coarfa, M Ittmann, R Fernandez-, JP Lydon
    Cytokine, 2019-06-18;123(0):154745.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  17. RIPK1 inhibition attenuates experimental autoimmune arthritis via suppression of osteoclastogenesis
    Authors: J Jhun, SH Lee, SY Kim, J Ryu, JY Kwon, HS Na, K Jung, SJ Moon, ML Cho, JK Min
    J Transl Med, 2019-03-15;17(1):84.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Soluble RANKL contributes to osteoclast formation in adult mice but not ovariectomy-induced bone loss
    Authors: J Xiong, K Cawley, M Piemontese, Y Fujiwara, H Zhao, JJ Goellner, CA O'Brien
    Nat Commun, 2018-07-25;9(1):2909.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Western Blot
  19. Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis
    Authors: X Zhang, HC Chiang, Y Wang, C Zhang, S Smith, X Zhao, SJ Nair, J Michalek, I Jatoi, M Lautner, B Oliver, H Wang, A Petit, T Soler, J Brunet, F Mateo, M Angel Puja, E Poggi, K Chaldekas, C Isaacs, BN Peshkin, O Ochoa, F Chedin, C Theoharis, LZ Sun, TJ Curiel, R Elledge, VX Jin, Y Hu, R Li
    Nat Commun, 2017-06-26;8(0):15908.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  20. Porphyromonas gingivalis Stimulates Bone Resorption by Enhancing RANKL (Receptor Activator of NF-kappaB Ligand) through Activation of Toll-like Receptor 2 in Osteoblasts.
    Authors: Kassem A, Henning P, Lundberg P, Souza P, Lindholm C, Lerner U
    J Biol Chem, 2015-06-17;290(33):20147-58.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Neutralization
  21. Autophagy regulator BECN1 suppresses mammary tumorigenesis driven by WNT1 activation and following parity.
    Authors: Cicchini M, Chakrabarti R, Kongara S, Price S, Nahar R, Lozy F, Zhong H, Vazquez A, Kang Y, Karantza V
    Autophagy, 2014-10-30;10(11):2036-52.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Fibromodulin and Biglycan Modulate Periodontium through TGFbeta/BMP Signaling.
    Authors: Wang L, Foster B, Kram V, Nociti F, Zerfas P, Tran A, Young M, Somerman M
    J Dent Res, 2014-06-25;93(8):780-787.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  23. The omega-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
    Authors: Wang J, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
    PLoS ONE, 2014-05-05;9(5):e96286.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  24. Paracrine-rescued lobulogenesis in chimeric outgrowths comprising progesterone-receptor-null mammary epithelium and redirected wild-type testicular cells.
    Authors: Bruno R, Boulanger C, Rosenfield S, Anderson L, Lydon J, Smith G
    J Cell Sci, 2013-11-04;127(0):27-32.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. CXCL5 stimulation of RANK ligand expression in Paget's disease of bone.
    Authors: Sundaram K, Rao D, Ries W, Reddy S
    Lab Invest, 2013-02-25;93(4):472-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Osteoclast differentiation factor RANKL controls development of progestin-driven mammary cancer.
    Authors: Schramek D, Leibbrandt A, Sigl V, Kenner L, Pospisilik JA, Lee HJ, Hanada R, Joshi PA, Aliprantis A, Glimcher L, Pasparakis M, Khokha R, Ormandy CJ, Widschwendter M, Schett G, Penninger JM
    Nature, 2010-09-29;468(7320):98-102.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse.
    Authors: Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP
    Dev. Biol., 2009-01-23;328(1):127-39.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice.
    Authors: Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL
    Circulation, 2008-01-02;117(3):411-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  29. Estrogen and progesterone are critical regulators of Stat5a expression in the mouse mammary gland.
    Authors: Santos SJ, Haslam SZ, Conrad SE
    Endocrinology, 2007-09-20;149(1):329-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  30. IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance.
    Authors: Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB
    J. Immunol., 2003-03-01;170(5):2655-62.
    Species: Mouse
    Sample Types: Synovial Fluid
    Applications: ELISA Development
  31. Naphthenic acid fraction components from oil sands process‐affected water from the Athabasca Oil Sands Region impair murine osteoblast differentiation and function
    Authors: Robert M. Gutgesell, Laiba Jamshed, Richard A. Frank, L. Mark Hewitt, Philippe J. Thomas, Alison C. Holloway
    Journal of Applied Toxicology
  32. Osteocyte Apoptosis Contributes to Cold Exposure-induced Bone Loss
    Authors: Jingke Du, Zihao He, Junqi Cui, Hanjun Li, Mingming Xu, Shuhong Zhang et al.
    Frontiers in Bioengineering and Biotechnology
  33. OXTR overexpression leads to abnormal mammary gland development in mice
    Authors: Dan Li, Yan Ji, Chunlan Zhao, Yapeng Yao, Anlan Yang, Honghong Jin et al.
    Journal of Endocrinology
  34. Circulating Extracellular Vesicles Express Receptor Activator of Nuclear Factor kappa B Ligand and Other Molecules Informative of the Bone Metabolic Status of Mouse Models of Experimentally Induced Osteoporosis
    Authors: Alfredo Cappariello, Maurizio Muraca, Anna Teti, Nadia Rucci
    Calcified Tissue International

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Mouse TRANCE/TNFSF11/RANK L Antibody
By Ju-Eun Hong on 03/13/2023
Application: IHC Sample Tested: Cartilage tissue Species: Mouse

doi.org/10.3390/ijms24021137

Immunohistochemistry Mouse TRANCE/TNFSF11/RANK L Antibody AF462
Mouse TRANCE/TNFSF11/RANK L Antibody
By Xue Yuan on 07/06/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Bone Species: Mouse
Immunocytochemistry/Immunofluorescence Mouse TRANCE/TNFSF11/RANK L Antibody AF462