Mouse TREM-1 Biotinylated Antibody

Catalog #: BAM1187 Datasheet / COA / SDS

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BAM1187

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Mouse TREM-1 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TREM-1 in direct ELISAs and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 174031

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TREM-1
Met1-Ser202
Accession # Q9JKE2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse TREM‑1 Fc Chimera (Catalog # 1187-TR)

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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TREM‑1 in Mouse Splenocytes. TREM-1 was detected in immersion fixed mouse splenocytes treated with LPS (left panel; positive staining) and untreated (right panel; negative staining) using Rat Anti-Mouse TREM-1 Biotinylated Monoclonal Antibody (Catalog # BAM1187) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

References

Bouchon, A. (2000) J. Immunol. 164:4991.
Bouchon, A. (2001) Nature 410:1103.
Nathan, C. and A. Ding (2001) Nature Med. 7:530.

Long Name

Triggering Receptor Expressed on Myeloid Cells 1

Entrez Gene IDs

54210 (Human); 58217 (Mouse)

Alternate Names

CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1