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Mouse TREM-1 PE-conjugated Antibody

Catalog #: FAB1187P
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1187P
Detection of TREM‑1 in Mouse Splenocytes by Flow Cytometry.
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Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TREM-1 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TREM-1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse TREM-2B, recombinant human (rh) TREM-1, or rhTREM-2 is observed.
Source
Monoclonal Rat IgG2A Clone # 174031
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM-1
Ala21-Ser202
Accession # Q9JKE2
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TREM-1 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of TREM‑1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB1037V) and either (A) Rat Anti-Mouse TREM-1 PE-conjugated Monoclonal Antibody (Catalog # FAB1187P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TREM-1

TREM-1 (Triggering Receptor Expressed on Myeloid cells) is a type I transmembrane protein having a single Ig-like domain. It associates with the adapter protein, DAP12, to deliver an activating signal. Several other TREM family members have been reported that are structurally similar but share less than 30% amino acid identity. TREM-1 is expressed on blood neutrophils and a subset of monocytes, and expression is up-regulated by bacterial LPS. Engagement of TREM-1 with a monoclonal antibody leads to expression of IL-8, MCP-1, and TNF-alpha suggesting that this receptor plays an important role in inflammatory responses. TREM-1 is expressed at high levels on neutrophils of patients with microbial sepsis and in mice with LPS-induced shock. Blockade of TREM-1 with a TREM-1/Fc fusion protein protected mice against LPS-induced shock.

References
  1. Bouchon, A. (2000) J. Immunol. 164:4991.
  2. Bouchon, A. (2001) Nature 410:1103.
  3. Nathan, C. and A. Ding (2001) Nature Med. 7:530.
Long Name
Triggering Receptor Expressed on Myeloid Cells 1
Entrez Gene IDs
54210 (Human); 58217 (Mouse)
Alternate Names
CD354 antigen; CD354; TREM1; TREM-1; TREM-1Triggering receptor expressed on monocytes 1; triggering receptor expressed on myeloid cells 1; triggering-receptor TREM1

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Citations for Mouse TREM-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Differential transcriptional profiles identify microglial- and macrophage-specific gene markers expressed during virus-induced neuroinflammation
    Authors: AB DePaula-Si, C Gorbea, DJ Doty, JE Libbey, JMS Sanchez, TJ Hanak, D Cazalla, RS Fujinami
    J Neuroinflammation, 2019-07-20;16(1):152.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. HIV-related proteins prolong macrophage survival through induction of Triggering receptor expressed on myeloid cells-1
    Authors: Z Yuan, X Fan, B Staitieh, C Bedi, P Spearman, DM Guidot, RT Sadikot
    Sci Rep, 2017-02-09;7(0):42028.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. TREM-1low is a novel characteristic for tumor-associated macrophages in lung cancer
    Oncotarget, 2016-06-28;7(26):40508-40517.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Triggering receptor expressed on myeloid cells 1 (TREM-1)-mediated Bcl-2 induction prolongs macrophage survival.
    Authors: Yuan Z, Syed M, Panchal D, Joo M, Colonna M, Brantly M, Sadikot R
    J Biol Chem, 2014-04-07;289(21):15118-29.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Stimulation of the molecule 4-1BB enhances host defense against Listeria monocytogenes infection in mice by inducing rapid infiltration and activation of neutrophils and monocytes.
    Authors: Lee SC, Ju SA, Sung BH, Heo SK, Cho HR, Lee EA, Kim JD, Lee IH, Park SM, Nguyen QT, Suh JH, Kim BS
    Infect. Immun., 2009-02-23;77(5):2168-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. TREM-1 promotes survival during septic shock in mice.
    Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
    Eur. J. Immunol., 2007-02-01;37(2):456-66.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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