Mouse TREM2 Biotinylated Antibody Summary
Leu19-Pro168
Accession # Q99NH8
Applications
This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) or Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729)
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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TREM‑2 in Mouse Splenocytes. TREM-2 was detected in immersion fixed mouse spleno-cytes using Mouse Anti-TREM-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Mouse TREM2 ELISA Standard Curve. Recombinant Human/Mouse TREM2 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
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Mouse TREM2 ELISA Standard Curve. Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM2
TREM2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types (1). It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC (2). A single human TREM2 gene has been described, however, two closely related orthologs were reported in mouse (3). The proteins differ by only three amino acids and were designated TREM2a and TREM2b. TREM2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail (1). It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals (4). Ligation of TREM2 on immature DC with anti-TREM2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts (5). In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM2 deficient individuals (6).
- Colonna, M. (2003) Nature Rev. Immunol. 3:445.
- Allcock, R. et al. (2003) Eur. J. Immunol. 33:567.
- Daws, M. et al. (2001) Eur. J. Immunol. 31:783.
- Bouchon, A. et al. (2001) J. Exp. Med. 194:1111.
- Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
- Cella, M. et al. (2003) J. Exp. Med. 198:645.
Product Datasheets
Citations for Mouse TREM2 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Rescue of a lysosomal storage disorder caused by Grn loss of function with a brain penetrant progranulin biologic
Authors: Logan T, Simon MJ, Rana A Et al.
Cell
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Trem2 deficiency attenuates breast cancer tumor growth in lean, but not obese or weight loss, mice and is associated with alterations of clonal T cell populations
Authors: Pierro, EW;Cottam, MA;An, H;Lehmann, BD;Pietenpol, JA;Wellen, KE;Makowski, L;Rathmell, JC;Fingleton, B;Hasty, AH;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Plasma
Applications: ELISA Detection -
BRI2-mediated regulation of TREM2 processing in microglia and its potential implications for Alzheimer's disease and related dementias
Authors: Yin, T;D'Adamio, L;
bioRxiv : the preprint server for biology
Species: Mouse, N/A, Transgenic Mouse
Sample Types: Tissue Homogenates, Protein
Applications: ELISA Capture -
Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome
Authors: X Shu, C Wei, WY Tu, K Zhong, S Qi, A Wang, L Bai, SX Zhang, B Luo, ZZ Xu, K Zhang, C Shen
Cell Reports, 2023-02-16;42(2):112133.
Species: Mouse
Sample Types: Cerebrospinal Fluid
Applications: ELISA Detection -
MS4A4A modifies the risk of Alzheimer disease by regulating lipid metabolism and immune response in a unique microglia state
Authors: SF You, L Brase, F Filipello, AK Iyer, J Del-Aguila, J He, R D'Oliveira, J Budde, J Norton, J Gentsch, NM Dräger, SM Sattler, M Kampmann, L Piccio, JC Morris, RJ Perrin, E McDade, Dominantly, SM Paul, AG Cashikar, BA Benitez, O Harari, CM Karch
medRxiv : the preprint server for health sciences, 2023-02-08;0(0):.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Trem2 H157Y increases soluble TREM2 production and reduces amyloid pathology
Authors: Wenhui Qiao, Yixing Chen, Jun Zhong, Benjamin J. Madden, Cristine M. Charlesworth, Yuka A. Martens et al.
Molecular Neurodegeneration
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Experimental evidence for temporal uncoupling of brain Abeta deposition and neurodegenerative sequelae
Authors: C Rother, RE Uhlmann, SA Müller, J Schelle, A Skodras, U Obermüller, LM Häsler, M Lambert, F Baumann, Y Xu, C Bergmann, G Salvadori, M Loos, I Brzak, D Shimshek, U Neumann, Dominantly, LC Walker, SA Schultz, JP Chhatwal, SA Kaeser, SF Lichtentha, M Staufenbie, M Jucker
Nature Communications, 2022-11-28;13(1):7333.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Capture -
Reduction of alphaSYN Pathology in a Mouse Model of PD Using a Brain-Penetrating Bispecific Antibody
Authors: S Roshanbin, U Julku, M Xiong, J Eriksson, E Masliah, G Hultqvist, J Bergström, M Ingelsson, S Syvänen, D Sehlin
Pharmaceutics, 2022-07-05;14(7):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Detection -
Novel App knock-in mouse model shows key features of amyloid pathology and reveals profound metabolic dysregulation of microglia
Authors: D Xia, S Lianoglou, T Sandmann, M Calvert, JH Suh, E Thomsen, J Dugas, ME Pizzo, SL DeVos, TK Earr, CC Lin, S Davis, C Ha, AW Leung, H Nguyen, R Chau, E Yulyanings, I Lopez, H Solanoy, ST Masoud, CC Liang, K Lin, G Astarita, N Khoury, JY Zuchero, RG Thorne, K Shen, S Miller, JJ Palop, D Garceau, M Sasner, JD Whitesell, JA Harris, S Hummel, J Gnörich, K Wind, L Kunze, A Zatcepin, M Brendel, M Willem, C Haass, D Barnett, TS Zimmer, AG Orr, K Scearce-Le, JW Lewcock, G Di Paolo, PE Sanchez
Molecular Neurodegeneration, 2022-06-11;17(1):41.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Capture -
Microglia use TAM receptors to detect and engulf amyloid &beta plaques
Authors: Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke
Nature Immunology, 2021-04-15;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Microglial Activation in the Right Amygdala-Entorhinal-Hippocampal Complex is Associated with Preserved Spatial Learning in AppNL-G-F mice
Authors: G Biechele, K Wind, T Blume, C Sacher, L Beyer, F Eckenweber, N Franzmeier, M Ewers, B Zott, S Lindner, FJ Gildehaus, B von Ungern, S Tahirovic, M Willem, P Bartenstei, P Cumming, A Rominger, J Herms, M Brendel
Neuroimage, 2020-12-29;0(0):117707.
Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA Development -
Trem2 promotes anti-inflammatory responses in microglia and is suppressed under pro-inflammatory conditions
Authors: Wenfei Liu, Orjona Taso, Rui Wang, Sevinc Bayram, Andrew C Graham, Pablo Garcia-Reitboeck et al.
Human Molecular Genetics
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Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice
Authors: VS Jadhav, PBC Lin, T Pennington, GV Di Prisco, AJ Jannu, G Xu, M Moutinho, J Zhang, BK Atwood, SS Puntambeka, SJ Bissel, AL Oblak, GE Landreth, BT Lamb
Mol Neurodegener, 2020-10-28;15(1):62.
Species: Mouse
Sample Types: Cell Lysates
Applications: ELISA Detection -
Plaque-associated myeloid cells derive from resident microglia in an Alzheimer’s disease model
Authors: Erin G. Reed-Geaghan, Andrew L. Croxford, Burkhard Becher, Gary E. Landreth
Journal of Experimental Medicine
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Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region
Authors: K Schlepckow, KM Monroe, G Kleinberge, L Cantuti-Ca, S Parhizkar, D Xia, M Willem, G Werner, N Pettkus, B Brunner, A Sülzen, B Nuscher, H Hampel, X Xiang, R Feederle, S Tahirovic, JI Park, R Prorok, C Mahon, CC Liang, J Shi, DJ Kim, H Sabelström, F Huang, G Di Paolo, M Simons, JW Lewcock, C Haass
EMBO Mol Med, 2020-03-10;0(0):e11227.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Capture -
Longitudinal PET Monitoring of Amyloidosis and Microglial Activation in a Second-Generation Amyloid-beta Mouse Model
Authors: Christian Sacher, Tanja Blume, Leonie Beyer, Finn Peters, Florian Eckenweber, Carmelo Sgobio et al.
Journal of Nuclear Medicine
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Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism
Authors: JK Götzl, M Brendel, G Werner, S Parhizkar, L Sebastian, G Kleinberge, AV Colombo, M Deussing, M Wagner, J Winkelmann, J Diehl-Schm, J Levin, K Fellerer, A Reifschnei, S Bultmann, P Bartenstei, A Rominger, S Tahirovic, ST Smith, C Madore, O Butovsky, A Capell, C Haass
EMBO Mol Med, 2019-06-01;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Capture -
Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model
Authors: L Zhong, Y Xu, R Zhuo, T Wang, K Wang, R Huang, D Wang, Y Gao, Y Zhu, X Sheng, K Chen, N Wang, L Zhu, D Can, Y Marten, M Shinohara, CC Liu, D Du, H Sun, L Wen, H Xu, G Bu, XF Chen
Nat Commun, 2019-03-25;10(1):1365.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection) -
The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism
Authors: G Kleinberge, M Brendel, E Mracsko, B Wefers, L Groeneweg, X Xiang, C Focke, M Deu beta ing, M Suárez-Cal, F Mazaheri, S Parhizkar, N Pettkus, W Wurst, R Feederle, P Bartenstei, T Mueggler, T Arzberger, I Knuesel, A Rominger, C Haass
EMBO J., 2017-05-30;0(0):.
Species: Mouse
Sample Types: CSF, Tissue Homogenates
Applications: ELISA Development, Immunoprecipitation -
Increase of TREM2 during Aging of an Alzheimer's Disease Mouse Model Is Paralleled by Microglial Activation and Amyloidosis
Authors: M Brendel, G Kleinberge, F Probst, A Jaworska, F Overhoff, T Blume, NL Albert, J Carlsen, S Lindner, FJ Gildehaus, L Ozmen, M Suárez-Cal, P Bartenstei, K Baumann, M Ewers, J Herms, C Haass, A Rominger
Front Aging Neurosci, 2017-01-31;9(0):8.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture), Western Blot -
The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia.
Authors: Sharif O, Gawish R, Warszawska J, Martins R, Lakovits K, Hladik A, Doninger B, Brunner J, Korosec A, Schwarzenbacher R, Berg T, Kralovics R, Colinge J, Mesteri I, Gilfillan S, Salmaggi A, Verschoor A, Colonna M, Knapp S
PLoS Pathog, 2014-06-12;10(6):e1004167.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
Triggering Receptor Expressed on Myeloid Cells-2 Correlates to Hypothermic Neuroprotection in Ischemic Stroke
Authors: Masahito Kawabori, Masaaki Hokari, Zhen Zheng, Jong Youl Kim, Cyrus Calosing, Christine L. Hsieh et al.
Therapeutic Hypothermia and Temperature Management
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DAP12 is required for macrophage recruitment to the lung in response to cigarette smoke and chemotaxis toward CCL2.
Authors: Koth LL, Cambier CJ, Ellwanger A
J. Immunol., 2010-04-26;184(11):6522-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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