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Mouse TROP-2 Biotinylated Antibody

Catalog #: BAF1122
4 Citations 1 Review Datasheet / COA / SDS
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BAF1122
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TROP-2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TROP-2 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) TROP-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TROP-2
Ser82-Thr268
Accession # Q8BGV3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TROP‑2
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse ovary

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TROP-2

TROP-2 is alternatively known as tumor-associated calcium signal transducer 2 and lymphocyte antigen 97.

Long Name
Tumor-associated Calcium Signal Transducer 2
Entrez Gene IDs
4070 (Human); 56753 (Mouse)
Alternate Names
Cell surface glycoprotein Trop-2; EGP1; EGP-1; epithelial glycoprotein-1; GA733-1; GA733-1GP50; gastrointestinal tumor-associated antigen GA7331; gp50; M1S1cell surface glycoprotein TROP2; Membrane component chromosome 1 surface marker 1; membrane component, chromosome 1, surface marker 1; Pancreatic carcinoma marker protein GA733-1; pancreatic carcinoma marker protein GA7331,40kD glycoprotein, identified by monoclonal GA733; T16; TACSTD2; TROP2; TROP-2; TROP2GA7331; tumor-associated calcium signal transducer 2

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Citations for Mouse TROP-2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Single-Cell Analysis Identifies LY6D as a Marker Linking Castration-Resistant Prostate Luminal Cells to Prostate Progenitors and Cancer
    Authors: JD Barros-Sil, DE Linn, I Steiner, G Guo, A Ali, H Pakula, G Ashton, I Peset, M Brown, NW Clarke, RT Bronson, GC Yuan, SH Orkin, Z Li, E Baena
    Cell Rep, 2018-12-18;25(12):3504-3518.e6.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Regulated proteolysis of Trop2 drives epithelial hyperplasia and stem cell self-renewal via beta-catenin signaling.
    Authors: Stoyanova T, Goldstein A, Cai H, Drake J, Huang J, Witte O
    Genes Dev, 2012-10-15;26(20):2271-85.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  3. Isolation, cultivation, and characterization of adult murine prostate stem cells
    Authors: Rita U. Lukacs, Andrew S. Goldstein, Devon A. Lawson, Donghui Cheng, Owen N. Witte
    Nature Protocols
  4. Trop2 identifies a subpopulation of murine and human prostate basal cells with stem cell characteristics.
    Authors: Goldstein AS, Lawson DA, Cheng D, Sun W, Garraway IP, Witte ON
    Proc. Natl. Acad. Sci. U.S.A., 2008-12-16;105(52):20882-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse TROP-2 Biotinylated Antibody

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Mouse TROP-2 Biotinylated Antibody
By Anonymous on 11/01/2021
Application: Flow Sample Tested: 4T1 mouse breast cancer cell line Species: Mouse

Detection of mouse TROP2 on the 4T1 breast cancer mouse cell line. 4T1 cells were treated with the Mouse TROP-2 Biotinylated antibody (catalog # BAF1122) (BLUE) or Normal Goat IgG biotinylated control antibody (catalog # BAF108) (RED), followed by streptavidin conjugated to APC.

Flow Cytometry Mouse TROP-2 Biotinylated Antibody BAF1122