Mouse Tryptase epsilon /BSSP-4 Biotinylated Antibody

Catalog #: BAM20591 Datasheet / COA / SDS

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BAM20591

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Tryptase epsilon /BSSP-4 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Tryptase epsilon /BSSP‑4 in ELISAs.

Source

Monoclonal Rat IgG_2A Clone # 246703

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Tryptase epsilon /BSSP‑4
Ala33-Ser306
Accession # Q9ER10

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

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Applications

Recommended Concentration

Sample

Mouse Tryptase epsilon /BSSP-4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Mouse Tryptase epsilon /BSSP‑4 Antibody (Catalog # MAB20592)

Standard: Recombinant Mouse Tryptase epsilon/BSSP-4 Protein, CF (Catalog # 2059-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase epsilon/BSSP-4

Tryptase epsilon, brain specific serine protease 4 (BSSP-4) and brain serine protease 2 (BSP-2) are different names given for the same serine protease that is encoded by the PRSS22 gene (1‑3). Initially identified having brain-specific expression, mouse Tryptase epsilon is preferentially expressed in epithelium-rich tissues such as the lung and eye, which is similar to its human counterpart (3). The mouse protein is synthesized with a signal peptide (amino acid residues 1 to 32), a pro peptide (residues 33 to 49) and a mature chain (residues 50 to 306) corresponding to the serine protease domain.

References

Wong, G.W. et al. (2001) J. Biol. Chem. 276:49169.
Davies, B.J. et al. (1998) J. Biol. Chem. 273:23004.
Wong, G.W. et al. (2004) J. Biol. Chem. 279:2438.

Entrez Gene IDs

64063 (Human); 70835 (Mouse)

Alternate Names

BSP-2; BSSP4; BSSP-4; hBSSP-4; MGC9599; prosemin; protease, serine S1 family member 22; protease, serine, 22; PRSS22; serine protease 22; serine protease 26; SP001LA; Tryptase epsilon; UNQ302/PRO343