Mouse TSPAN8 APC-conjugated Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TSPAN8 in CMT‑93 Mouse Cell Line by Flow Cytometry. CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 APC-conjugated Monoclonal Antibody (Catalog # FAB6524A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
Background: TSPAN8
Tetraspanin-8 (TSPAN8), also known as TM4SF3 and human tumor-associated antigen CO-0029, is a member of the transmembrane 4 (tetraspanin) superfamily. It is a cell surface 27 - 34 kDa protein with intracellular N- and C-termini, 4 transmembrane segments and a large extracellular loop (LEL) domain from aa 106-206. TSPAN8 is involved in integrin-mediated cell motility, metastasis, cell proliferation and differentiation, and is over-expressed in carcinomas including hepatocellular carcinoma and colon cancer. Polymorphisms have variably been associated with type II diabetes, bipolar disorder and schizophrenia. Human TSPAN8 shares 70% aa sequence identity with mouse and rat TSPAN8.
Product Datasheets
Citations for Mouse TSPAN8 APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Combined multidimensional single-cell protein and RNA profiling dissects the cellular and functional heterogeneity of thymic epithelial cells
Authors: Klein, F;Veiga-Villauriz, C;Börsch, A;Maio, S;Palmer, S;Dhalla, F;Handel, AE;Zuklys, S;Calvo-Asensio, I;Musette, L;Deadman, ME;White, AJ;Lucas, B;Anderson, G;Holländer, GA;
Nature communications
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive
Authors: NY Fu, AC Rios, B Pal, CW Law, P Jamieson, R Liu, F Vaillant, F Jackling, KH Liu, GK Smyth, GJ Lindeman, ME Ritchie, JE Visvader
Nat. Cell Biol, 2017-02-13;19(3):164-176.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Tumor collection/processing under physioxia uncovers highly relevant signaling networks and drug sensitivity
Authors: B Kumar, AK Adebayo, M Prasad, ML Capitano, R Wang, P Bhat-Naksh, M Anjanappa, E Simpson, D Chen, Y Liu, JM Schilder, AB Colter, C Maguire, CJ Temm, G Sandusky, EH Doud, AB Wijeratne, AL Mosley, HE Broxmeyer, H Nakshatri
Science Advances, 2022-01-12;8(2):eabh3375.
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Gives two clear populations which look very distinct. Clear recommendation.
