Mouse TSPAN8 PE-conjugated Antibody Summary
Accession # Q8R3G9
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of TSPAN8 in CMT‑93 Mouse Cell Line by Flow Cytometry. CMT-93 mouse rectal carcinoma cell line was stained with Rat Anti-Mouse TSPAN8 PE-conjugated Monoclonal Antibody (Catalog # FAB6524P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
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Background: TSPAN8
TSPAN8 (Tetraspanin-8), also known as TM4SF3 and CO-029, is a 27-33 kDa member of the tetraspanin family of proteins. As evidenced by its name, it is a 4-transmembrane (glyco)protein that is found on/in multiple tumor types as well as smooth and skeletal muscle, endothelium and hematopoietic progenitor cells. All 4-transmembrane proteins are not tetraspanins. Tetraspanins are characterized by the presence of a very short intracellular C- and N-terminus, and a very long extracellular (or LEL) domain linking the 3rd and 4th transmembrane segments. On TSPAN8 this domain is 98 amino acids (aa) in length and represents 42% of the entire length of mouse TSPAN8 (or 235 aa). TSPAN8, like other tetraspanins, forms the backbone of a network that allows molecule interactions on (or in) plasma membranes. This ability is dependent upon molecule palmitoylation and creates a microdomain (or TEM) that is distinct from typical membrane rafts, the latter of which is characterized by the presence of GPI-linked proteins and the molecule caveolin. Within this TEM, TSPAN8 may form homodimers, and is known to interact with multiple molecule types, including CD151, CD81, CD9, CD13, EpCAM, EWI-F, MMP-9, Meprin-beta, Rictor, TACE and the integrins alpha 3(CD49c) beta 1, alpha 6 beta 4 and alpha 4(CD49d) beta 1. Such interactions appear to positively or negatively modulate individual molecule activity. These interactions are generally described within the context of exosomes, 30-100 nm membrane-bound vesicles that are released from multiple cell types. TSPAN8-containing exosomes are reported to either facilitate cell motility or act as an antiapoptotic agent for target cells. Over aa 106-203 constituting the LEL, mouse TSPAN8 shares 58% and 73% aa sequence identity with human and rat TSPAN8, respectively.
Product Datasheets
Citations for Mouse TSPAN8 PE-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Sequential genome-wide CRISPR-Cas9 screens identify genes regulating cell-surface expression of tetraspanins
Authors: J Yang, F Guo, HS Chin, GB Chen, CH Ang, Q Lin, W Hong, NY Fu
Cell Reports, 2023-01-31;42(2):112065.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Identification of quiescent and spatially restricted mammary stem cells that are hormone responsive
Authors: NY Fu, AC Rios, B Pal, CW Law, P Jamieson, R Liu, F Vaillant, F Jackling, KH Liu, GK Smyth, GJ Lindeman, ME Ritchie, JE Visvader
Nat. Cell Biol, 2017-02-13;19(3):164-176.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
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