Mouse Uromodulin Antibody Summary
Ser24-Ala618
Accession # Q91X17
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of Mouse Uromodulin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Uromodulin at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
View Larger
Uromodulin in Mouse Kidney. Uromodulin was detected in immersion fixed frozen sections of mouse kidney using Rat Anti-Mouse Uromodulin Monoclonal Antibody (Catalog # MAB5175) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to convoluted tubule epithelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Uromodulin
Uromodulin (also Tamm-Horsfall glycoprotein or THP) is a 105‑120 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑148), a ZP domain that mediates oligomerization (aa 335‑590), and a cleavable C-terminal propeptide (aa 619‑642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17 aa substitution for aa 601‑642, and a 166 aa substitution for aa 441‑642. Over aa 25‑618, mouse Uromodulin shares 78% and 89% aa identical to human and rat Uromodulin, respectively.
Product Datasheets
Citations for Mouse Uromodulin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
Filter your results:
Filter by:
-
Androgen action on renal calcium and phosphate handling: Effects of bisphosphonate treatment and low calcium diet
Authors: Khalil R, Simitsidellis I, Kim NR et al.
Molecular and Cellular Endocrinology
-
Tubule-Specific Mst1/2 Deficiency Induces CKD via YAP and Non-YAP Mechanisms.
Authors: Xu, C, Wang, L Et al.
J Am Soc Nephrol
-
ZEB2 controls kidney stromal progenitor differentiation and inhibits abnormal myofibroblast expansion and kidney fibrosis
Authors: S Kumar, X Fan, H Milo Rasou, R Sharma, DJ Salant, W Lu
JCI Insight, 2023-01-10;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
T cell deficiency precipitates antibody evasion and emergence of neurovirulent polyomavirus
Authors: MD Lauver, G Jin, KN Ayers, SN Carey, CS Specht, CS Abendroth, AE Lukacher
Elife, 2022-11-07;11(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Androgen action on renal calcium and phosphate handling: Effects of bisphosphonate treatment and low calcium diet
Authors: Khalil R, Simitsidellis I, Kim NR et al.
Molecular and Cellular Endocrinology
-
Tolvaptan activates the Nrf2/HO-1 antioxidant pathway through PERK phosphorylation
Authors: T Fujiki, F Ando, K Murakami, K Isobe, T Mori, K Susa, N Nomura, E Sohara, T Rai, S Uchida
Sci Rep, 2019-06-25;9(1):9245.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development
Authors: N Naiman, K Fujioka, M Fujino, MT Valerius, SS Potter, AP McMahon, A Kobayashi
Dev. Cell, 2017-05-22;41(4):349-365.e3.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Missense Mutation of POU Domain Class 3 Transcription Factor 3 in Pou3f3L423P Mice Causes Reduced Nephron Number and Impaired Development of the Thick Ascending Limb of the Loop of Henle
PLoS ONE, 2016-07-15;11(7):e0158977.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Botulinum toxin A complex exploits intestinal M cells to enter the host and exert neurotoxicity.
Authors: Matsumura, Takuhiro, Sugawara, Yo, Yutani, Masahiro, Amatsu, Sho, Yagita, Hideo, Kohda, Tomoko, Fukuoka, Shin-Ich, Nakamura, Yutaka, Fukuda, Shinji, Hase, Koji, Ohno, Hiroshi, Fujinaga, Yukako
Nat Commun, 2015-02-17;6(0):6255.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse Uromodulin Antibody
Average Rating: 5 (Based on 2 Reviews)
Have you used Mouse Uromodulin Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
- loaded 30 ug of total protein from mouse kidney lysate
- wet transfer (100V, 1h)
- used buffers as described by datasheet
- 1:1500 dilution of UMOD antibody in 0.5% BSA
- 1:10000 dilution of rat IgG antibody (HAF005) in 3% BSA
