Mouse USP2 Antibody Summary
Lys47-Met396
Accession # NP_932759
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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USP2 in NIH‑3T3 Mouse Cell Line. USP2 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Goat Anti-Mouse USP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5804) at 0.3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Detection of Mouse USP2 by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse USP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5804) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for USP2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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USP2 in Mouse Kidney. USP2 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse USP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5804) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in glandular epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: USP2
USP2 (Ubiquitin-specific-processing protease 2; also Ubiquitin carboxy-terminal hydrolase 2) is a cytosolic member of the peptidase C19 family of enzymes. It is widely expressed and serves to deubiquitinate select target substrates. USP2 effects are determined by its targets. In cells where it increases Mdm2, p53 is degraded and apoptosis is blocked. In cells where it increases Itch concentration, Flip activity is interrupted and apoptosis is promoted. USP2 is reported to form homo‑oligomers. There are at least two isoform variants. Each is approximately 70 kDa in size, with one showing a 266 aa, and another a 272 aa substitution for aa 1‑49. Over aa 47‑396, mouse USP2 shares 96% and 99% aa identity with human and rat USP2, respectively.
Product Datasheets
Citations for Mouse USP2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Dysregulation and oncogenic activities of ubiquitin specific peptidase 2a in the pathogenesis of hepatocellular carcinoma
Authors: Zhang, X;Nadolny, C;Chen, Q;Ali, W;Hashmi, SF;Deng, R;
American journal of cancer research
Species: Human, Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma
Authors: C Nadolny, X Zhang, Q Chen, SF Hashmi, W Ali, C Hemme, N Ahsan, Y Chen, R Deng
American journal of cancer research, 2021-10-15;11(10):4746-4767.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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