Mouse Vitamin D BP Antibody

Catalog #: MAB4188 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4188-100
MAB4188-SP

Mouse Vitamin D BP ELISA Standard Curve.

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All Vitamin D BP Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Vitamin D BP Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Vitamin D BP in direct ELISAs.

Source

Monoclonal Rat IgG₁ Clone # 884704

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Vitamin D BP
Met1-Ser476
Accession # P21614

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB41881).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse Vitamin D BP DuoSet ELISA Kit (Catalog # DY4188-05) for convenient development of a sandwich ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Mouse Vitamin D BP ELISA Standard Curve. Recombinant Mouse Vitamin D BP protein was serially diluted 2-fold and captured by Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB41881) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Vitamin D BP Monoclonal Antibody (Catalog # MAB4188) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vitamin D BP

VDBP (Vitamin D binding protein; also group-specific component and GC-globulin) is a 52‑58 kDa, monomeric glycoprotein member of the ALB/AFP/VDB family of molecules. It is found in blood, urine and CSF, carries Vitamin D and its metabolites, and serves as an actin-scavenging protein. Mature mouse VDBP is 460 amino acids (aa) in length. It contains three albumin-type domains (aa 26‑476) that are accompanied by 14 intrachain disulfide bonds. There are three potential alternative splice forms. One shows a deletion of aa 346‑421, a second shows a 67 aa substitution for aa 345‑421, and a third shows a 34 aa substitution for aa 346‑423. All these variants involve the second and third albumin-like domains. Mature mouse VDBP (aa 17‑476) is 77% and 90% aa identical to human and rat VDBP, respectively.

Long Name

Vitamin D Binding Protein

Entrez Gene IDs

2638 (Human); 14473 (Mouse); 24384 (Rat)

Alternate Names

DBP; DBP/GC; DBP-MAF; DBPVDBG; EC 6.3.1.5; Gc; gc-globulin; Gc-MAF; GRD3; group-specific component (vitamin D binding protein); Group-specific component; hDBP; VDB; VDBG; VDBP; Vitamin D BP; vitamin D-binding alpha-globulin; vitamin D-binding protein