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Mouse VLDLR Antibody

Catalog #: AF2258
19 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2258
AF2258-SP
VLDL R in Mouse Embryo.
2 Images
Product Details
Citations (19)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VLDLR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VLDL R in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VLDL R
Thr25-Ala798
Accession # AAA59384
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse VLDL R (Catalog # 2258-VL)
Immunohistochemistry
3-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry VLDL R antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

VLDL R in Mouse Embryo. VLDL R was detected in immersion fixed frozen sections of mouse embryo using Mouse VLDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2258) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunohistochemistry VLDL R antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

VLDL R in Mouse Kidney. VLDL R was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse VLDL R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2258) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes of convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VLDLR

VLDL R is a 105 kDa type I integral membrane protein that belongs to the LDL receptor family. It plays a significant role in lipid metabolism and in nervous system development and function (1, 2). Mouse VLDL R has a 770 amino acid (aa) extracellular domain (ECD) and a 54 aa cytoplasmic region. The ECD contains eight LDLR class A repeats, three EGF-like repeats, six LDLR class B repeats, and a juxtamembrane region that is rich in O-linked glycosylation (3, 4). The cytoplasmic domain contains one NPXY internalization motif. VLDL R is predominantly expressed in striated muscle, adipose tissue, brain, and endothelial cells lining capillaries and small arterioles (3-6). VLDL R participates in the tissue uptake of fatty acids from plasma by mediating the internalization of ApoE-containing lipoparticles (i.e. VLDL, beta ‑VLDL, and chylomicron remnants) (5, 7). VLDL R binds and internalizes lipoprotein lipase (LPL) and mediates its transport from the basolateral to the lumenal face of endothelial cells (6, 8). VLDL R knockout mice are characterized by reduced LPL activity, reduced serum triglyceride clearance, and a resistance to developing obesity (7, 9, 10). VLDL R influences breast cancer cell motility by mediating the uptake of uPAR-PAI1 complexes (6, 11). Lipoprotein accumulation via macrophage VLDL R is instrumental in promoting the formation of atherosclerotic plaques (12). In the nervous system, VLDL R and ApoE R2 interactions with Reelin are critical for neuronal migration and positioning in the developing brain (13). VLDL R also functions in adult hippocampal synapse maturation, synaptic plasticity, and memory formation (14, 15). The ECD of mouse VLDL R shares 95% aa sequence identity with human and rat VLDL R. Within shared regions, mouse VLDL R shares 55% and 53% aa sequence identity with ApoE R2 and LDL R, respectively.

References
  1. Qiu, S. et al. (2006) Neurobiol. Learn. Mem. 85:16.
  2. May, P. et al. (2005) Cell. Mol. Life Sci. 62:2325.
  3. Gafvels, M.E. et al. (1994) Endocrinology 135:387.
  4. Oka, K. et al. (1994) Eur. J. Biochem. 224:975.
  5. Wyne, K.L. et al. (1996) Arterioscler. Thromb. Vasc. Biol. 16:407.
  6. Argraves, K.M. et al. (1995) J. Biol. Chem. 270:26550.
  7. Goudriaan, J.R. et al. (2001) Arterioscler. Thromb. Vasc. Biol. 21:1488.
  8. Obunike, J.C. et al. (2001) J. Biol. Chem. 276:8934.
  9. Yagyu, H. et al. (2002) J. Biol. Chem. 277:10037.
  10. Goudriaan, J.R. et al. (2004) J. Lipid Res. 45:1475.
  11. Webb, D.J. et al. (1999) J. Biol. Chem. 274:7412.
  12. van Eck, M. et al. (2005) Atherosclerosis 183:230.
  13. Jossin, Y. et al. (2004) J. Neurosci. 24:514.
  14. Niu, S. et al. (2004) Neuron 41:71.
  15. Weeber, E.J. et al. (2002) J. Biol. Chem. 277:39944.
Long Name
Very Low Density Lipoprotein Receptor
Entrez Gene IDs
7436 (Human); 22359 (Mouse)
Alternate Names
CARMQ1; CHRMQ1; FLJ35024; very low density lipoprotein receptor; very low-density lipoprotein receptor; VLDL R; VLDL receptor; VLDLR; VLDL-R; VLDLRCH

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Citations for Mouse VLDLR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. The Secreted Glycoprotein Reelin Suppresses the Proliferation and Regulates the Distribution of Oligodendrocyte Progenitor Cells in the Embryonic Neocortex
    Authors: Himari Ogino, Tsuzumi Nakajima, Yuki Hirota, Kohki Toriuchi, Mineyoshi Aoyama, Kazunori Nakajima et al.
    The Journal of Neuroscience
  2. SIRT3 deficiency exacerbates fatty liver by attenuating the HIF1 alpha -LIPIN 1 pathway and increasing CD36 through Nrf2.
    Authors: Barroso, E, RodrIguez-RodrIguez, R Et al.
    Cell Commun Signal
  3. VLDLR is not essential for reelin-induced neuronal aggregation but suppresses neuronal invasion into the marginal zone
    Authors: Yuki Hirota, Kazunori Nakajima
    Development
  4. Very low-density lipoprotein receptor increases in a liver-specific manner due to protein deficiency but does not affect fatty liver in mice
    Authors: Y Oshio, Y Hattori, H Kamata, Y Ozaki-Masu, A Seki, Y Tsuruta, A Takenaka
    Scientific Reports, 2021-04-13;11(1):8003.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Reelin Can Modulate Migration of Olfactory Ensheathing Cells and Gonadotropin Releasing Hormone Neurons via the Canonical Pathway
    Authors: L Dairaghi, E Flannery, P Giacobini, A Saglam, H Saadi, S Constantin, F Casoni, BW Howell, S Wray
    Front Cell Neurosci, 2018-08-03;12(0):228.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Mathematical model of early Reelin-induced Src family kinase-mediated signaling
    Authors: H Hass, F Kipkeew, A Gauhar, E Bouché, P May, J Timmer, HH Bock
    PLoS ONE, 2017-10-19;12(10):e0186927.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Pericyte loss influences Alzheimer-like neurodegeneration in mice.
    Authors: Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B
    Nat Commun, 2013-01-01;4(0):2932.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Blocking
  8. Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation.
    J. Cell Biol., 2012-06-04;197(6):837-49.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. miR-200c regulates FGFR-dependent epithelial proliferation via Vldlr during submandibular gland branching morphogenesis.
    Authors: Rebustini IT, Hayashi T, Reynolds AD
    Development, 2011-11-24;139(1):191-202.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  10. Exercise during pregnancy mitigates Alzheimer-like pathology in mouse offspring.
    Authors: Herring A, Donath A, Yarmolenko M, Uslar E, Conzen C, Kanakis D, Bosma C, Worm K, Paulus W, Keyvani K
    FASEB J., 2011-09-24;26(1):117-28.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. apoE isoform-specific disruption of amyloid beta peptide clearance from mouse brain.
    Authors: Deane R, Sagare A, Hamm K, Parisi M, Lane S, Finn MB, Holtzman DM, Zlokovic BV
    J. Clin. Invest., 2008-11-13;118(12):4002-13.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Bovine lactoferrin inhibits Japanese encephalitis virus by binding to heparan sulfate and receptor for low density lipoprotein.
    Authors: Chien YJ, Chen WJ, Hsu WL, Chiou SS
    Virology, 2008-07-21;379(1):143-51.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Expression of VLDLR in the retina and evolution of subretinal neovascularization in the knockout mouse model's retinal angiomatous proliferation.
    Authors: Hu W, Jiang A, Liang J, Meng H, Chang B, Gao H, Qiao X
    Invest. Ophthalmol. Vis. Sci., 2008-01-01;49(1):407-15.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Disabled 1 Is Part of a Signaling Pathway Activated by Epidermal Growth Factor Receptor
    Authors: Paula Dlugosz, Magdalena Teufl, Maximilian Schwab, Katharina Eva Kohl, Johannes Nimpf
    International Journal of Molecular Sciences
  15. Differential Action of Reelin on Oligomerization of ApoER2 and VLDL Receptor in HEK293 Cells Assessed by Time-Resolved Anisotropy and Fluorescence Lifetime Imaging Microscopy.
    Authors: Dlugosz Paula, Tresky Roland, Nimpf Johannes
    Frontiers in Immunology
  16. Hepatic regulation of VLDL receptor by PPAR beta /δ and FGF21 modulates non-alcoholic fatty liver disease
    Authors: Mohammad Zarei, Emma Barroso, Xavier Palomer, Jianli Dai, Patricia Rada, Tania Quesada-López et al.
    Molecular Metabolism
  17. Xanthine oxidoreductase is involved in macrophage foam cell formation and atherosclerosis development.
    Authors: Kushiyama A, Okubo H, Sakoda H, Kikuchi T, Fujishiro M, Sato H, Kushiyama S, Iwashita M, Nishimura F, Fukushima T, Nakatsu Y, Kamata H, Kawazu S, Higashi Y, Kurihara H, Asano T.
    Arterioscler Thromb Vasc Biol;32(2):291-8.
  18. A low-carbohydrate ketogenic diet induces the expression of very-low-density lipoprotein receptor in liver and affects its associated metabolic abnormalities
    Authors: Tetsuya Okuda
    npj Science of Food
  19. Reelin-Nrp1 Interaction Regulates Neocortical Dendrite Development in a Context-Specific Manner
    Authors: Takao Kohno, Keisuke Ishii, Yuki Hirota, Takao Honda, Makoto Makino, Takahiko Kawasaki et al.
    The Journal of Neuroscience

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