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Mouse WIF-1 Antibody

Catalog #: MAB135 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB135-050
MAB135-500
MAB135-SP
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse WIF-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse WIF-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human WIF-1 is observed.
Source
Monoclonal Rat IgG2A Clone # 134714
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse WIF-1
Gly29-Trp379
Accession # Q9WUA1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse WIF‑1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: WIF-1

WIF-1 is a secreted protein of approximately 50 kDa that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers. Mature mouse WIF-1 shares 95% and 98% amino acid sequence identity with human and rat WIF-1, respectively.

Long Name
Wnt Inhibitory Factor
Entrez Gene IDs
11197 (Human); 24117 (Mouse)
Alternate Names
WIF1; WIF-1; WNT inhibitory factor 1

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