Mouse Wnt-9b Antibody

Catalog #: MAB36691 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB36691-100
MAB36691-SP

Alkaline Phosphatase Production Induced by Wnt‑9b and Neutralization by Mouse Wnt‑9b Antibody.

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Mouse Wnt-9b Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Wnt-9b in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 964742

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Wnt-9b
Met1-Arg359
Accession # O35468

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Neutralization

Measured by its ability to neutralize Wnt‑9b-induced alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 125-750 ng/mL in the presence of 400 ng/mL Recombinant Mouse Wnt‑9b.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Alkaline Phosphatase Production Induced by Wnt‑9b and Neutralization by Mouse Wnt‑9b Antibody. Recombinant Mouse Wnt-9b (Catalog # 3669-WN) induces alkaline phosphatase production in the C3H10T1/2 mouse embryonic fibroblast in a dose-dependent manner (orange line). Alkaline Phosphatase Production elicited by Recombinant Mouse Wnt-9b (400 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Wnt-9b Monoclonal Antibody (Catalog # MAB36691). The ND₅₀ is typically 125-750 ng/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-9b

Mouse Wnt-9b, previously called Wnt-14b or Wnt-15, is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes (1‑3). Wnts mainly transduce signals by binding to receptors of the Frizzled family, in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6) (1, 2). The 359 aa mouse Wnt-9b precursor contains a 336 aa mature region with 24 conserved cysteines (3). Mature mouse Wnt-9b shares 93%, 98%, 92%, 92% and 91% aa identity with human, rat, canine, equine and bovine Wnt-9b, respectively. It is evolutionarily related to Wnt-9a and Wnt-3, which share 63% and 32% aa identity, respectively (4). Wnt-9b mRNA is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver (3, 4). It appears to direct mesenchymal-to-epithelial transition in the kidney and other tissues (5). During kidney development, it is expressed in the ureteric bud and induces mesonephric and metanephric tubulogenesis, nephron development, and caudal extension of the Mullerian duct, acting upstream of Wnt-4 (5‑7). Induction is dependent on beta -catenin activity, implicating the canonical signaling pathway (7). Mice devoid of Wnt-9b die shortly after birth due to kidney agenesis, while low expression results in small kidneys with fewer nephrons (1, 5, 6). Mutations of Wnt-9b also cause incompletely penetrant cleft lip and palate in mice, indicating its involvement with facial midline morphogenesis (8, 9). It has weak transforming activity compared to other transforming Wnts (4).

References

http://www.stanford.edu/group/nusselab/cgi-bin/wnt/.
Logan, C. Y. and R. Nusse (2004) Annu. Rev. Cell Dev. Biol. 20:781.
Kirikoshi, H. and M. Katoh (2002) Int. J. Mol. Med. 9:135.
Qian, J. et al. (2003) Genomics 81:34.
Carroll, T. J. et al. (2005) Dev. Cell 9:283.
Merkel, C. E. et al. (2007) Pediatr. Nephrol. 22:1825.
Park, J-S. et al. (2007) Development 134:2533.
Lan, Y. et al. (2006) Dev. Dyn. 235:1448.
Juriloff, D. M. and M. J. Harris (2008) Birth Defects Res. A Clin. Mol. Teratol. 82:63.

Long Name

Wingless-type MMTV Integration Site Family, Member 9b

Entrez Gene IDs

7484 (Human); 22412 (Mouse); 303586 (Rat)

Alternate Names

Protein Wnt-14b; wingless-type MMTV integration site family, member 15; wingless-type MMTV integration site family, member 9B; Wnt-14b; WNT14Bprotein Wnt-9b; Wnt-15; WNT15Protein Wnt-15; Wnt9b; Wnt-9b