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Mouse XL Cytokine Luminex® Performance 45-plex Fixed Panel

Catalog #: LKTM015
2 Citations Datasheet / COA / SDS
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LKTM015

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Luminex Mouse Cytokine Panel Standard Curves
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Mouse XL Cytokine Luminex® Performance 45-plex Fixed Panel Summary

Assay Type bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and antibody-coated beads
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

  • Simultaneously profile up to 50 analytes of your choice in one sample
  • Choose from a wide menu of analytes, including many unique analytes
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-4 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate

Please allow additional time for international orders.

Kit Components
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Standard/Sample Diluent
  • Wash Buffer
  • Streptavidin-PE
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Certificate of Analysis**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Precision

Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay

Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 40 different serum assays.
  n=40 (20x2 levels) n=30
Analyte Intra-Assay (%CV) Inter-Assay (%CV)
BAFF/BLyS/TNFSF13B 1.6 11.8
CCL2/JE/MCP-1 2.1 11.9
CCL3/MIP-1 alpha 2.1 11.0
CCL4/MIP-1 beta 1.5 6.8
CCL5/RANTES 2.6 9.7
CCL11/Eotaxin 2.8 12.3
CCL19/MIP-3 beta 2.3 11.1
Chitinase 3-like 1 2.4 16.7
CXCL1/KC 1.9 96
CXCL10/IP-10/CRG-2 2.5 12.4
EGF 2.0 11.7
FGF-basic 2.1 10.4
Flt-3 ligand 1.9 12.1
G-CSF 2.6 12.1
GDF-15 2.1 12.0
GM-CSF 2.3 11.6
ICAM-1 1.9 12.6
IFN-gamma 2.1 9.2
IL-1 alpha/IL-1F1 2.2 10.7
IL-1 beta/IL-1F3 1.9 11.9
IL-2 1.7 12.5
IL-3 2.1 11.2
IL-4 2.3 13.0
IL-5 5.6 10.8
IL-6 2.0 11.3
IL-7 2.9 14.5
IL-9 1.7 7.3
IL-10 1.7 11.7
IL-11 2.4 13.8
IL-12 p70 2.8 12.2
IL-13 3.0 14.2
IL-16 1.7 10.5
IL-17A 1.4 11.4
IL-18 5.1 14.8
IL-21 3.3 16.1
IL-27 2.2 9.7
IL-31 2.5 14.1
LDL R 2.5 11.2
LIF 2.0 12.9
LIX 2.4 11.0
M-CSF 2.1 11.6
TIMP-1 1.9 8.9
TNF-alpha 1.6 9.9
VEGF 2.1 11.9

Accuracy

Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk.

Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
  Linearity Spiked
Recovery
Analyte N Average % N Average %
BAFF/BLyS/TNFSF13B* 4 95 4 HE
CCL2/JE/MCP-1 4 109 4 98
CCL3/MIP-1 alpha 4 105 4 107
CCL4/MIP-1 beta 4 119 4 85
CCL5/RANTES 4 144 4 76
CCL11/Eotaxin* 4 82 4 HE
CCL19/MIP-3 beta 4 99 4 107
Chitinase 3-like 1* 4 89 4 HE
CXCL1/KC 4 109 4 96
CXCL10/IP-10/CRG-2 4 108 4 104
EGF 4 148 4 67
FGF-basic 4 133 4 86
Flt-3 ligand 4 104 4 104
G-CSF 4 ◊ 4 ◊
GDF-15 4 ◊ 4 ◊
GM-CSF 4 128 4 86
ICAM-1 4 131 4 88
IFN-gamma 4 153 4 56
IL-1 alpha/IL-1F1 4 136 4 76
IL-1 beta/IL-1F2 4 115 4 97
IL-1ra/IL-1F3 4 145 4 64
IL-2 4 112 4 96
IL-3 4 165 4 53
IL-4 4 136 4 67
IL-5 4 99 4 101
IL-6 4 120 4 91
IL-7 4 105 4 104
IL-9 4 119 4 98
IL-10 4 145 4 65
IL-11 4 ◊ 4 ◊
IL-12 p70 4 113 4 88
IL-13 4 119 4 56
IL-16 3 135 3 86
IL-17A 4 116 4 92
IL-18 4 ◊ 4 ◊
IL-21 4 109 4 91
IL-27 4 158 4 50
IL-31 4 136 4 70
LDL R 4 108 4 104
LIF 4 ◊ 4 ◊
LIX 4 ◊ 4 ◊
M-CSF 4 117 4 100
TIMP-1 4 99 4 119
TNF-alpha 4 109 4 97
VEGF 4 130 4 78
"◊" Not recommended for particular sample type, see Samples Collection section for reference.
*Natural linearity
"HE" Spiked recovery was not observed due to natural linearity in high endogenous samples.
 
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

Scientific Data

N/A Luminex Mouse Cytokine Panel Standard Curves View Larger

Luminex Mouse Cytokine Panel Standard Curves

N/A Luminex Mouse Cytokine Panel Serum Sample Values View Larger

Luminex Mouse Cytokine Panel Serum Sample Values

N/A View Larger

Antigens were spiked in at 3-times the value of the high standard tested for cross-reactivity within the panel.   < 0.5% cross-reactivity was measured across all 45 analytes for all antigens.

Product Datasheets

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Product Specific Notices
Luminex® is a registered trademark of Luminex Corporation.

Citations for Mouse XL Cytokine Luminex® Performance 45-plex Fixed Panel

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Diquat exacerbates oxidative stress and neuroinflammation by blocking the autophagic flux of microglia in the hippocampus
    Authors: Wang, P;Song, CY;Lu, X;Zhou, JN;Lin, LY;Li, T;Zhang, Q;Lu, YQ;
    Ecotoxicology and environmental safety
    Species: Mouse
    Sample Types: Serum
  2. VEXAS syndrome is characterized by inflammasome activation and monocyte dysregulation
    Authors: Kosmider, O;Possémé, C;Templé, M;Corneau, A;Carbone, F;Duroyon, E;Breillat, P;Chirayath, TW;Oules, B;Sohier, P;Luka, M;Gobeaux, C;Lazaro, E;Outh, R;Le Guenno, G;Lifermann, F;Berleur, M;Le Mene, M;Friedrich, C;Lenormand, C;Weitten, T;Guillotin, V;Burroni, B;Boussier, J;Willems, L;Aractingi, S;Dionet, L;Tharaux, PL;Vergier, B;Raynaud, P;Ea, HK;Ménager, M;Duffy, D;Terrier, B;
    Nature communications
    Species: Human
    Sample Types: Plasma

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