Mouse ZNRF3 Antibody Summary
Lys53-Met216
Accession # Q5SSZ7
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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ZNRF3 in HEK Transfectants. ZNRF3 was detected in immersion fixed HEK human embryonic kidney cell transfectants using Rabbit Anti-Mouse ZNRF3 Monoclonal Antibody (Catalog # MAB8328) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZNRF3
ZNRF3 (Zinc/RING Finger protein 3) is a member of the ZNRF3 family of type 1 transmembrane ubiquitin ligases (1). Mature mouse ZNRF3 consists of a 163 amino acid (aa) extracellular domain (ECD) with a protease associated (PA) domain fold, a short transmembrane segment, and a 675 aa cytoplasmic tail that contains a RING-type zinc finger with E3 ubiquitin ligase activity. Within the ECD, mouse and human ZNRF3 share 98% sequence identity. ZNRF3 is co-expressed on the cell surface with the homologous protein RNF43. Both proteins serve to inhibit the Wnt signaling pathway through the ubiquitination of LRP6 and a majority of the Frizzled family of Wnt receptors (1, 4, 6). ZNRF3 ubiquitin ligase activity is regulated jointly by R-Spondin and its cell surface receptors, Lgr4, 5, and 6 (2). The R-Spondin-Lgr complex binds to and facilitates the removal of the ZNRF3 from the plasma membrane, resulting in an enhancement of Wnt signaling (3, 4). Conversely, ZNRF3 can antagonize R-Spondin enhanced Wnt signaling (3, 5). ZNRF3 is highly expressed in crypt stem cells of the intestine where it modulates Wnt-induced cell proliferation to control the turnover of intestinal epithelial cells (6). ZNRF3 expression is down-regulated in gastric carcinomas, and ZNRF3 mutations are linked to carcinomas of the gastric tract, pancreas, liver, and ovary (7-10).
- de Lau, W. et al. (2014) Genes Dev. 28:305.
- Xie, Y. et al. (2013) EMBO Rep. 14:1120.
- Peng, W.C. et al. (2013) PLoS One 8:e83110.
- Hao, H.X. et al. (2012) Nature 485:195.
- Moad, H.E. and A.A. Pioszak (2013) Biochemistry 52:7295.
- Koo, B.K. et al. (2012) Nature 488:665.
- Ong, C.K. et al. (2012) Nat. Genet. 44:690.
- Zhou, Y. et al. (2013) J. Mol. Histol. 44:555.
- Ryland, G.L. et al. (2013) J. Pathol. 229:469.
- Jiang, X. et al. (2013) Proc. Natl. Acad. Sci. USA 110:12649.
Product Datasheets
Product Specific Notices
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.FAQs
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