N21-MAX Insulin Free Media Supplement (50X)

For the reliable maturation and superior long-term maintenance of primary neurons under insulin free culture conditions.

Why Culture Neurons in Serum-Free N21-MAX Media Supplement?

The N21-MAX Insulin Free Media Supplement improves upon the traditional B27¹ and NS21² neuronal supplements, offering a serum-free, insulin-free, and fully-defined formulation that is optimized for the reliable maturation, consistent health, and superior function of neurons in culture. This insulin-free formulation is ideal insulin-sensitive research, including studies focused on insulin secretion or insulin receptor function.

N21-MAX was designed to eliminate uncontrolled variables, such as those found in both serum-containing media and in some commercial serum-free neuronal supplements. Use of undefined media supplements can confound neuronal growth and maturation. N21-MAX contains a cocktail of factors optimized for neuronal survival, neurite extension, and synaptic maturation. Each lot of N21-MAX is tested on primary hippocampal neurons to ensure consistent performance for the user.

The N21-MAX Insulin Free Media Supplement:

• Is fully defined and quality tested to reduce unwanted experimental variation.
• Contains factors qualified to support reproducible and long-term neuron culture.
• Modified from the published B27 formulation¹.
• Does not require an astrocyte feeder layer.
• Has been tested to support the viability and growth of E18 rat hippocampal neurons.

1. Brewer et al., (1993) J. Neurosci. Res. 32:567
2. Chen et al., (2008) J. Neurosci. Methods 171:239

N21-MAX Insulin Free Media Supplement Components

N21-MAX Insulin Free Media Supplement is optimized for the maturation and long-term culturing of neuronal cells. The supplement is supplied as a 50X concentrated solution and contains the following 20 components:

• Albumin (bovine)
• L-Carnitine
• Catalase
• Corticosterone
• Ethanolamine
• Glutathione
• Galactose
• Holo-Transferrin
• Linoleic Acid
• Lipoic Acid
• Progesterone
• Putrescine
• Retinyl acetate
• Retinol
• Selenite
• Superoxide dismutase
• Triiodo-L-thyronine
• D,L-alpha-Tocopherol
• D,L-alpha-Tocopherol acetate

Supplied in a volume sufficient to supplement 500 mL of media at the recommended concentration. N21-MAX Insulin Free Media Supplement is tested for use in Neurobasal Media.

Precautions

This product contains human transferrin. This transferrin was purified from donor plasma and tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen.