What is the immunogen for the NanoLuc® (Nluc) Luciferase Antibody, Catalog # MAB10026? Does this antibody recognize only LgBiT and not SmBiT?

Name: NanoLuc® (Nluc) Luciferase Antibody
Brand: R&D Systems
SKU: MAB10026
Rating: 4.5 (2 reviews)

The immunoogen for Catalog # MAB10026 is Synthetic peptide of NanoLuc® (Nluc) Luciferase. For more details on the compound, Promega Corporation should be contacted. NanoBit (LgBit+SmBit) is a derivative of the original product NanoLuc, where portion of the protein is split to get two complementary peptides. This antibody detects LgBiT and not SmBiT.

NanoLuc® (Nluc) Luciferase Antibody

Catalog #: MAB10026
4 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB10026-100
MAB10026-SP

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Detection of NanoLuc® Luciferase and Large BiT by Western Blot.

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Product Details

Citations (4)

FAQs

Supplemental Products

Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

NanoLuc® (Nluc) Luciferase Antibody Summary

Specificity

Detects NanoLuc® (Nluc) Luciferase in direct ELISAs. Detects NanoLuc® (Nluc) Luciferase and Large BiT in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 965808

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Synthetic peptide of NanoLuc® (Nluc) Luciferase

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of NanoLuc® Luciferase and Large BiT by Western Blot. Western blot shows Recombinant Large BiT and Recombinant NanoLuc®. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human NanoLuc® (Nluc) Luciferase Monoclonal Antibody (Catalog # MAB10026) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for NanoLuc® and Large BiT at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Luciferase

NanoLuc® (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. NanoLuc® Binary Technology (NanoBiT™) is a two subunit system based on NanoLuc® luciferase. Large BiT (LgBiT; 17.6kDa) is the large subunit of the NanoBiT® system.

References

Alternate Names

ec 1.13.12.7; firefly luciferase; Fluc; LuC; Luciferase; luciferin 4 monooxygenase; Luciferin 4-monooxygenase

Product Datasheets

Product Datasheet

COA

SDS

Product Specific Notices

NanoLuc and NanoBiT are registered trademarks of Promega Corporation.

Citations for NanoLuc® (Nluc) Luciferase Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins
Authors: James H. Thierer, Stephen C. Ekker, Steven A. Farber
Nature Communications
Real-time tracking of bioluminescent influenza A virus infection in mice
Authors: JH Kim, H Bryant, E Fiedler, T Cao, JO Rayner
Scientific Reports, 2022-02-24;12(1):3152.
Species: Canine
Sample Types: Cell Lysates
Applications: Western Blot
PAX8 and MECOM are interaction partners driving ovarian cancer
Authors: M Bleu, F Mermet-Mei, V Apfel, L Barys, L Holzer, M Bachmann S, R Lopes, I Amorim Mon, C Delmas, A Hinniger, S Chau, M Kaufmann, S Haenni, K Berneiser, M Wahle, I Moravec, A Vissières, T Poetsch, E Ahrné, N Carte, J Voshol, E Bechter, J Hamon, M Meyerhofer, D Erdmann, M Fischer, T Stachyra, F Freuler, S Gutmann, C Fernández, T Schmelzle, U Naumann, G Roma, K Lawrenson, C Nieto-Ober, A Cobos-Corr, S Ferretti, D Schübeler, GG Galli
Nature Communications, 2021-04-26;12(1):2442.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
A point mutation decouples the lipid transfer activities of microsomal triglyceride transfer protein
Authors: MH Wilson, S Rajan, A Danoff, RJ White, MR Hensley, VH Quinlivan, R Recacha, JH Thierer, FJ Tan, EM Busch-Nent, L Ruddock, MM Hussain, SA Farber
PLoS Genet., 2020-08-06;16(8):e1008941.
Species: Zebrafish
Sample Types: Tissue Homogenates
Applications: Western Blot

FAQs

What is the immunogen for the NanoLuc® (Nluc) Luciferase Antibody, Catalog # MAB10026? Does this antibody recognize only LgBiT and not SmBiT?
- The immunoogen for Catalog # MAB10026 is Synthetic peptide of NanoLuc® (Nluc) Luciferase. For more details on the compound, Promega Corporation should be contacted. NanoBit (LgBit+SmBit) is a derivative of the original product NanoLuc, where portion of the protein is split to get two complementary peptides. This antibody detects LgBiT and not SmBiT.

View all Antibody FAQs