NIR Dye s775z, NHS
Chemical Name: 2-((1E,3Z,5E)-4-(2,6-bis((4-(2,5,8,11-tetraoxadodecyl)-1H-1,2,3-triazol-1-yl)methyl)-4-(((2,5-dioxopyrrolidin-1-yl)oxy)carbonyl)phenyl)-7-((E)-3,3-dimethyl-5-sulfonato-1-(3-(trimethylammonio)propyl)indolin-2-ylidene)hepta-1,3,5-trien-1-yl)-3,3-dimethyl-1-(3-(trimethylammonio)propyl)-3H-indol-1-ium-5-sulfonate chloride
Purity: ≥90%
Biological Activity
Key information: NIR Dye s775z, NHS is a near-infrared (NIR) fluorescent dye supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for in vivo imaging.Application: Fluorescence microscopy, in vivo imaging.
Properties and Photophysical Data: When conjugated to antibodies, NIR Dye s775z, NHS is substantially brighter and more photostable than competitor dyes. It retains excellent target specificity, even when the degree of labeling (DOL) is very high. Excitation and emission maxima (λ) are 775 nm and 795 nm, respectively; quantum yield = 0.09; extinction coefficient = 201,000 M-1cm-1; A280 correction factor = 0.11.
For further information about how NIR Dye s775z, NHS may be used, please see our protocol: Conjugation Protocol for Amine Reactive Dyes.
Scientific Data
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Application of NIR Dye s775z, NHS in HT-1080 human fibrosarcoma. HT-1080 cells were fixed, co-incubated with primary rabbit anti-tubulin overnight at 4 °C. The following day, they were incubated with secondary goat anti-rabbit labelled with NIR Dye s775z (green) for 2 h at room temperature. Cells were co-stained with Hoechst 33342 (blue, Catalog # 5117, Tocris). Dye used at 1:100 dilution. Scale: 30 μm. For more information on this work read Schreiber et al (2021) High-Performance Near-Infrared Fluorescent Secondary Antibodies for Immunofluorescence. Anal.Chem. 93 3643 PMID: 33566567
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Application of NIR Dye s775z, NHS in vivo. Mock surgery process of a tumor bearing mouse 3h post retro-orbital co-injection of 100 μL (20 nmol) NIR Dye s775z, NHS conjugated to integrin receptor targeting cRGDfK peptide. Images were taken before the surgery, and after excising the tumor from mouse body. For more information on this work read Gamage et al (2021) Comparison of cRGDfK Peptide Probes with Appended Shielded Heptamethine Cyanine Dye (s775z) for Near Infrared Fluorescence Imaging of Cancer. ACS Omega 6 30130 PMID: 34778684
Optical Data for NIR Dye s775z, NHS
| Emission Color | Near-IR |
|---|---|
| Brightness | |
| λabs | 775 nm |
| λem | 795 nm |
| Extinction Coefficient (ε) | 201000 M-1cm-1 |
| Quantum Yield (φ) | 0.09 |
| Closest Laser line | 750 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Correction Factor 280 | 0.11 |
| Application | Fluorescence Microscopy, In Vivo Imaging |
| Alternative For | FNIR-Tag, IRDye® 800CW, UL766 |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectral Viewer
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Additional Information
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