Non-Human Primate CCL19/MIP-3b Cartridge

Catalog #: SPCKB-NP-011294 Datasheet / COA / SDS
Simple Plex Non-Human Primate MIP-3b assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-NP-011294
SPCKA-NP-011294
SPCKC-NP-011294
Control Products Available
Non-Human Primate CCL19/MIP-3b Ella Assay Standard Curve
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Non-Human Primate CCL19/MIP-3b Cartridge Summary

Assay Type
Automated sandwich ELISA
Format
Microfluidic cartridge containing three Glass Nano Reactors (GNR)
Assay Length
90 minutes
Sample Type & Volume Required Per Well*
Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
Sensitivity
0.35 pg/mL
Assay Range
3.9 - 6,000 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant MIP-3 beta. 
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of non-human primate Macrophage Inflammatory Protein 3 beta (MIP-3 beta ) in serum and plasma (EDTA/Heparin).

Recovery

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 86 79-101
Heparin Plasma (n=5) 82 78-88
Serum (n=5) 81 76-89

Linearity

Samples containing and/or spiked with high concentrations of MIP-3 beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.
Non-Human Primate CCL19/MIP-3b Ella Assay Linearity

Scientific Data

Non-Human Primate CCL19/MIP-3b Ella Assay Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL19/MIP-3 beta

Macrophage inflammatory protein 3 beta (MIP-3 beta), also known as ELC (EBI1-ligand chemokine) and exodus-3, is constitutively expressed in various lymphoid tissues, including thymus, lymph nodes, appendix and spleen. It is distantly related to other CC chemokines (20-30% amino acid sequence identity).

Entrez Gene IDs:
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names:
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

FAQs

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