Normal Sheep IgG Fluorescein Control

Catalog # Availability Size / Price Qty
IC016F
Detection of Sheep IgG Isotype Control by Flow Cytometry
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Normal Sheep IgG Fluorescein Control Summary

Specificity
Serum was obtained from naive (non-immunized) sheep.
Source
Polyclonal Sheep IgG
Purification
Protein A or G purified
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P(fluorochrome/protein) ratio of R&D Systems specific polyclonal antibodies. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Detection of Sheep IgG Control by Flow Cytometry HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868F, filled histogram) or Sheep IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Normal Sheep IgG Fluorescein Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Regulatory T Cells Play a Role in a Subset of Idiopathic Preterm Labor/Birth and Adverse Neonatal Outcomes
    Authors: N Gomez-Lope, M Arenas-Her, R Romero, D Miller, V Garcia-Flo, Y Leng, Y Xu, J Galaz, SS Hassan, CD Hsu, H Tse, C Sanchez-To, B Done, AL Tarca
    Cell Rep, 2020-07-07;32(1):107874.
  2. The common parasite Toxoplasma gondii induces prostatic inflammation and microglandular hyperplasia in a mouse model.
    Authors: Colinot DL, Garbuz T, Bosland MC et al.
    Prostate

FAQs

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Reviews for Normal Sheep IgG Fluorescein Control

Average Rating: 5 (Based on 1 Review)

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Normal Sheep IgG Fluorescein Control
By Anonymous on 08/24/2017
Application: Flow Sample Tested: Adult splenocytes Species: Mouse

Isotype control