Normal Sheep IgG Fluorescein Control Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Sheep IgG Control by Flow Cytometry HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human/Mouse Arginase 1/ARG1 Fluorescein-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC5868F, filled histogram) or Sheep IgG Fluorescein-conjugated Isotype Control Antibody (Catalog # IC016F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Preparation and Storage
Background: IgG
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Normal Sheep IgG Fluorescein Control
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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Regulatory T Cells Play a Role in a Subset of Idiopathic Preterm Labor/Birth and Adverse Neonatal Outcomes
Authors: N Gomez-Lope, M Arenas-Her, R Romero, D Miller, V Garcia-Flo, Y Leng, Y Xu, J Galaz, SS Hassan, CD Hsu, H Tse, C Sanchez-To, B Done, AL Tarca
Cell Rep, 2020-07-07;32(1):107874.
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The common parasite Toxoplasma gondii induces prostatic inflammation and microglandular hyperplasia in a mouse model.
Authors: Colinot DL, Garbuz T, Bosland MC et al.
Prostate
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Isotype control