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NucA nuclease Antibody

Catalog #: MAB100634 1 Review Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB100634-100
MAB100634-SP
NucA nuclease ELISA Standard Curve.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Product Datasheets Related Research Areas

NucA nuclease Antibody Summary

Specificity
Detects NucA nuclease in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1028917
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived S. marcescens NucA nuclease
Met1-Asn266
Accession # P13717
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100635).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA View Larger

NucA nuclease ELISA Standard Curve. Recombinant NucA nuclease protein was serially diluted 2-fold and captured by Mouse Anti-NucA nuclease Monoclonal Antibody (MAB100635) coated on a Clear Polystyrene Microplate (DY990). Mouse Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100634) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NucA nuclease

Long Name
NucA nuclease
Entrez Gene IDs
23387471 (Bacterial)
Alternate Names
DcD; NucA nuclease

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FAQs

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Reviews for NucA nuclease Antibody

Average Rating: 5 (Based on 1 Review)

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Benzonase® Nuclease/NucA Antibody
By Anonymous on 02/27/2023
Application: ELISA Sample Tested: Cell Lysates Species: Human

The antibody works super well, with very clear and strong signal in ELISA format.

ELISA Benzonase® Nuclease/NucA Antibody MAB100634