NucA nuclease Antibody Summary
Met1-Asn266
Accession # P13717
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of NucA nuclease by Western Blot. Western blot shows recombinant NucA nuclease protein. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100631) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for NucA nuclease at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of NucA nuclease by Simple WesternTM. Simple Western lane view shows recombinant NucA nuclease protein, loaded at 0.2 mg/mL. A specific band was detected for NucA nuclease at approximately 35 kDa (as indicated) using 10 µg/mL of Rabbit Anti-NucA nuclease Monoclonal Antibody (Catalog # MAB100631). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NucA nuclease
Product Datasheets
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