Nucleic Acid Dye Green I

Biological Activity

Nucleic Acid Dye Green I is an asymmetric cyanine dye that specifically binds to dsDNA. Frequently used dye in qPCR and is further used as a stain for in-gel visualization of DNA. Also used in the detection of amplification products in Loop-Mediated Isothermal Amplification (LAMP) assays; this method has been used for the detection of SARS-CoV-2 virus. When bound to dsDNA the fluorescence intensity is 1000-fold greater than when in the unbound form. Does not require washing or de-staining steps. Compatible with all commonly used fluorescence detection systems. Excitation maximum = 495 nm; emission maximum = 520 nm.

This product is sold as a DMSO stock.

Usage Information

• Supplied as a 10,000x concentrate in DMSO.
• Soluble in TE (10 mM Tris-HCl, 1 mM EDTA, pH 8.0), TBE, or TAE buffer.
• Verify that the pH of the staining solution, at the temperature used for staining, is between 7.5 and 8.0 (preferably pH 8.0).
• Use diluted samples within 24 hours to ensure maximal sensitivity.

Optical Data for Nucleic Acid Dye Green I

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. SYBR Green I DNA staining increases the detection sensitivity of viruses by polymerase chain reaction.
   Karlsen et al.
   J.Virol.Methods, 1995;55:153
2. High-throughput real-time quantitative reverse transcription PCR.
   Bookout et al.
   Curr.Protoc.Mol.Biol., 2006;:doi: 10.1002/0471142
3. Rapid detection of Zika virus in urine samples and infected mosquitos by Reverse Transcription-Loop-Mediated Isothermal Amplification.
   Lamb et al.
   Sci.Rep., 2018;8:3803
4. Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification
   Lamb et al.
   PLoS One, 2020;15:e0234682

Product Datasheets