OG 488, SE
Discontinued Product
Chemical Name: 2',7'-Difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid 2,5-dioxo-1-pyrrolidinyl ester
Biological Activity
OG 488, SE is a green fluorescent dye. pH-insensitive in physiological pH range (pKa = 4.6). Can be used to monitor pH change in acidic organelles or pathways. Excitation/emission λ 501/526 nm.Optical Data for OG 488, SE
| Emission Color | Green |
|---|---|
| Brightness | |
| λabs | 496 nm |
| λem | 516 nm |
| Extinction Coefficient (ε) | 76,000 M-1cm-1 |
| Quantum Yield (φ) | 0.9 |
| Closest Laser line | 488 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| pH Sensitivity | pH-insensitive in physiological pH range (pKa = 4.6) |
| Correction Factor 280 | 0.12 |
| Application | Imaging, acidic organelle probe, flow, STED |
| Alternative For | TFAX 488, SE, 5(6)-SFX (Fluorescein), SE, FITC, |
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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Phagosomal pH determination by dual fluorescence flow cytometry.
Vergene et al.
Anal.Biochem., 1998;255:127
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