Oxidized PTP Active Site Antibody

Detection of Oxidized PTP by Western Blot. Western blot shows oxidized PTP immunoprecipitated from lysates of HepG2 human hepatocellular carcinoma cell line using Rat Anti-SHP-2 antibody. PVDF membrane was probed with 1 µg/mL Mouse Anti-Oxidized PTP Active Site Monoclonal Antibody (Catalog # MAB2844) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Oxidized SHP-2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Detection of Human Oxidized PTP Active Site by Western Blot Topoisomerase inhibitors induce JAK2-STAT1-CXCL1 and migration through ROS. a Relative DCFH-DA levels in SW480 and SW620 cells treated with VP-16 (V, 20 μM), ADM (A, 0.2 μg/ml), or CPT-11 (C, 80 μg/ml) for 0.5 h. P, a positive control with Rosup H2O2 (50 μg/ml, 0.5 h). (B) Western blot of oxidized PTPs after treatement with VP-16 (20 μM), ADM (0.2 μg/ml), or CPT-11 (80 μg/ml) for 0.5 h. c Confirmation of VP-16-induced PTP1B oxidization. After treatment with VP-16 (20 μM, 0.5 h), cell lysates (200 μg per sample) were immunoprecipitated with 1 μg anti-PTP1B or pre-immune IgG for 12 h. Precipitates and cell lysates (input, 50 μg per sample) were analyzed by Western blot with anti-oxPTP and anti-PTP1B. d Western blot of JAK2 and STAT1 phosphorylation and CXCL1 expression in cells pretreated with GSH (10 mM) for 2 h and subsequently treated with VP-16 (20 μM) or CPT-11 (80 μg/ml) for 0.5 h. e Migration assay of cells treated with GSH (10 mM) plus VP-16 (20 μM) or CPT-11 for 24 h Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31438997), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Human Oxidized PTP Active Site by Western Blot Topoisomerase inhibitors induce JAK2-STAT1-CXCL1 and migration through ROS. a Relative DCFH-DA levels in SW480 and SW620 cells treated with VP-16 (V, 20 μM), ADM (A, 0.2 μg/ml), or CPT-11 (C, 80 μg/ml) for 0.5 h. P, a positive control with Rosup H2O2 (50 μg/ml, 0.5 h). (B) Western blot of oxidized PTPs after treatement with VP-16 (20 μM), ADM (0.2 μg/ml), or CPT-11 (80 μg/ml) for 0.5 h. c Confirmation of VP-16-induced PTP1B oxidization. After treatment with VP-16 (20 μM, 0.5 h), cell lysates (200 μg per sample) were immunoprecipitated with 1 μg anti-PTP1B or pre-immune IgG for 12 h. Precipitates and cell lysates (input, 50 μg per sample) were analyzed by Western blot with anti-oxPTP and anti-PTP1B. d Western blot of JAK2 and STAT1 phosphorylation and CXCL1 expression in cells pretreated with GSH (10 mM) for 2 h and subsequently treated with VP-16 (20 μM) or CPT-11 (80 μg/ml) for 0.5 h. e Migration assay of cells treated with GSH (10 mM) plus VP-16 (20 μM) or CPT-11 for 24 h Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31438997), licensed under a CC-BY license. Not internally tested by R&D Systems.