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PAR/pADPr Antibody

Catalog #: 4335-MC-100
64 Citations 3 Reviews Datasheet / COA / SDS
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4335-MC-100
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Detection of Human PAR/pADPr by Western Blot.
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Citations (64)
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PAR/pADPr Antibody Summary

Specificity
The antibody is specific for PAR polymers 2 to 50 units long, but does not recognize structurally related RNA, DNA, ADP-ribose monomers, NAD, or other nucleic acid monomers.
Source
Monoclonal Mouse IgG3 Clone # 10HA
Purification
Protein A or G purified from ascites
Immunogen
Purified ADP-ribose polymers between 2 and 50 units long
Formulation
Supplied as 100 μL of a 0.2 μm filtered solution in TBS, at a concentration of 1 mg/mL
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
Jurkat human acute T cell leukemia cell line treated with H2O2
ELISA
As determined by testing.
 
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of human kidney
Immunoprecipitation
As determined by testing.
 
Immunoaffinity Purification
As determined by testing.
 
Immunocytochemistry
3-25 µg/mL
Immersion fixed 786-O human renal cell adenocarcinoma cell line
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Jurkat human acute T cell leukemia cell line treated with 1 mM H2O2 for 5 minutes

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human PAR/pADPr by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 2 mM H2O2 for 5 minutes. PVDF membrane was probed with 1:1000 dilution of Mouse Anti-PAR/pADPr Monoclonal Antibody (Catalog # 4335-MC-100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for PAR polymers, as indicated. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Intracellular Staining by Flow Cytometry View Larger

Detection of PAR in Jurkat cells by Flow Cytometry. Jurkat human acute T cell leukemia cell line treated with 1 mM H2O2 for 5 minutes (filled histogram) or resting (open histogram) were stained with Mouse Anti-PAR Monoclonal Antibody (Catalog # 4335-MC-100) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Immunohistochemistry View Larger

Detection of PAR/pADPr in Human Kidney. PAR/pADPr was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-PAR/pADPr Monoclonal Antibody (Catalog # 4335-MC-100) at 1.7 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry View Larger

Detection of PAR/pADPr in 786‑O Human Cell Line. PAR/pADPr was detected in immersion fixed 786‑O human renal cell adenocarcinoma cell line untreated (negative) or treated (positive) with 2mM hydrogen peroxide for 5 minutes using Mouse Anti-PAR/pADPr Monoclonal Antibody (Catalog # 4335-MC-100) at 3 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus upon stimulation with hydrogen peroxide. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: PAR/pADPr

PARP [Poly(ADP-ribose) Polymerase], also known as ADPRT and PPOL, is a 118-kDa enzyme that uses NAD as a substrate to catalyze the covalent transfer of ADP-ribose to a variety of nuclear protein acceptors. ADP ribosyltransferase is required for cellular repair, and PARP expression is induced by single-strand breaks in DNA. PARP is proteolytically cleaved by Caspase-3 into two fragments of 89- and 24-kDa in one of the hallmark events of apoptosis.

Long Name
Poly [ADP-ribose] Polymer
Alternate Names
pADPr; PAR; Poly(ADP-ribose)

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Citations for PAR/pADPr Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

64 Citations: Showing 1 - 10
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  1. PARP1-catalyzed PARylation of YY1 mediates endoplasmic reticulum stress in granulosa cells to determine primordial follicle activation
    Authors: Chen, W;E, Q;Sun, B;Zhang, P;Li, N;Fei, S;Wang, Y;Liu, S;Liu, X;Zhang, X;
    Cell death & disease
  2. ALS-FUS mutations cause abnormal PARylation and histone H1.2 interaction, leading to pathological changes
    Authors: Alirzayeva, H;Loureiro, R;Koyuncu, S;Hommen, F;Nabawi, Y;Zhang, WH;Dao, TTP;Wehrmann, M;Lee, HJ;Vilchez, D;
    Cell reports
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  3. Heme oxygenase-1 protects cells from replication stress
    Authors: Chudy, P;Kochan, J;Wawro, M;Nguyen, P;Gorczyca, M;Varanko, A;Retka, A;Ghadei, SS;Napieralska, E;Grochot-Prz?czek, A;Szade, K;Berendes, LS;Park, J;Soko?owski, G;Yu, Q;Józkowicz, A;Nowak, WN;Krzeptowski, W;
    Redox biology
    Species: N/A
    Sample Types: Recombinant Protein
    Applications: Western Blot
  4. Ku70 Binding to YAP Alters PARP1 Ubiquitination to Regulate Genome Stability and Tumorigenesis
    Authors: Shu, Y;Jin, X;Ji, M;Zhang, Z;Wang, X;Liang, H;Lu, S;Dong, S;Lin, Y;Guo, Y;Zhuang, Q;Wang, Y;Lei, Z;Guo, L;Meng, X;Zhou, G;Zhang, W;Chang, L;
    Cancer research
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunocytochemistry
  5. The TDRD3-USP9X complex and MIB1 regulate TOP3B homeostasis and prevent deleterious TOP3B cleavage complexes
    Authors: Saha, S;Huang, SN;Yang, X;Saha, LK;Sun, Y;Khandagale, P;Jenkins, LM;Pommier, Y;
    Nature communications
    Species: Human
    Sample Types: Cell Lysates
    Applications: Westen Blot
  6. Spatiotemporal roles of AMPK in PARP-1- and autophagy-dependent retinal pigment epithelial cell death caused by UVA
    Authors: Wu, AY;Sekar, P;Huang, DY;Hsu, SH;Chan, CM;Lin, WW;
    Journal of biomedical science
    Species: Human
    Sample Types:
  7. The three-dimensional structure of the EBV genome plays a crucial role in regulating viral gene expression in EBVaGC
    Authors: Maestri, D;Napoletani, G;Kossenkov, A;Preston-Alp, S;Caruso, LB;Tempera, I;
    Nucleic acids research
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Nimbolide targets RNF114 to induce the trapping of PARP1 and synthetic lethality in BRCA-mutated cancer
    Authors: Li, P;Zhen, Y;Kim, C;Liu, Z;Hao, J;Deng, H;Deng, H;Zhou, M;Wang, XD;Qin, T;Yu, Y;
    Science advances
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. O-GlcNAc has crosstalk with ADP-ribosylation via PARG
    Authors: Li, J;Liu, X;Peng, B;Feng, T;Zhou, W;Meng, L;Zhao, S;Zheng, X;Wu, C;Wu, S;Chen, X;Xu, X;Sun, J;Li, J;
    The Journal of biological chemistry
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Exploring a new mechanism between lactate and VSMC calcification: PARP1/POLG/UCP2 signaling pathway and imbalance of mitochondrial homeostasis
    Authors: Zhu, Y;Zhang, JL;Yan, XJ;Ji, Y;Wang, FF;
    Cell death & disease
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. A bi-functional PARP-HDAC inhibitor with activity in Ewing sarcoma
    Authors: Louise Ramos, Sarah Truong, Beibei Zhai, Jay Joshi, Fariba Ghaidi, Michael M. Lizardo et al.
    Clinical Cancer Research
  12. Alkylation of nucleobases by 2-chloro-N,N-diethylethanamine hydrochloride (CDEAH) sensitizes PARP1-deficient tumors
    Authors: Minwoo Wie, Keon Woo Khim, Arnold S Groehler IV, Soomin Heo, Junhyeok Woo, Kook Son et al.
    NAR Cancer
  13. Fatty acid oxidation facilitates DNA double-strand break repair by promoting PARP1 acetylation
    Authors: Yang, S;Hwang, S;Kim, B;Shin, S;Kim, M;Jeong, SM;
    Cell death & disease
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. The Poly (ADP-ribose) polymerase inhibitor olaparib and pan-ErbB inhibitor neratinib are highly synergistic in HER2 overexpressing epithelial ovarian carcinoma in vitro and in vivo
    Authors: C Han, B McNamara, S Bellone, J Harold, P Manara, TMP Hartwich, L Mutlu, Y Yang-Hartw, M Zipponi, C Demirkiran, MS Verzosa, G Altwerger, E Ratner, GS Huang, M Clark, V Andikyan, M Azodi, PR Dottino, PE Schwartz, AD Santin
    Gynecologic oncology, 2023-01-25;170(0):172-178.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. The ADP-ribose hydrolase NUDT5 is important for DNA repair
    Authors: H Qi, RH Grace Wrig, M Beato, BD Price
    Cell Reports, 2022-12-20;41(12):111866.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. PARP-inhibition reprograms macrophages toward an anti-tumor phenotype
    Authors: L Wang, D Wang, O Sonzogni, S Ke, Q Wang, A Thavamani, F Batalini, SA Stopka, MS Regan, S Vandal, S Tian, J Pinto, AM Cyr, VC Bret-Moune, G Baquer, HP Eikesdal, M Yuan, JM Asara, YJ Heng, P Bai, NYR Agar, GM Wulf
    Cell Reports, 2022-10-11;41(2):111462.
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  17. Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells
    Authors: L Abdelghany, T Kawabata, S Goto, K Jingu, TS Li
    Translational Oncology, 2022-10-04;26(0):101548.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  18. Integrated proteomics identifies PARP inhibitor-induced pro-survival signaling changes as potential vulnerabilities in ovarian cancer
    Authors: O Deng, S Dash, TC Nepomuceno, B Fang, SY Yun, EA Welsh, HR Lawrence, D Marchion, JM Koomen, AN Monteiro, U Rix
    The Journal of Biological Chemistry, 2022-09-29;0(0):102550.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Lagging strand gap suppression connects BRCA-mediated fork protection to nucleosome assembly through PCNA-dependent CAF-1 recycling
    Authors: T Thakar, A Dhoonmoon, J Straka, EM Schleicher, CM Nicolae, GL Moldovan
    Nature Communications, 2022-09-09;13(1):5323.
    Species: Human
    Sample Types: Whole Cells
    Applications: Proximity Ligation Assay
  20. Pgc-1alpha controls epidermal stem cell fate and skin repair by sustaining NAD+ homeostasis during aging
    Authors: W Wong, ED Crane, H Zhang, J Li, TA Day, AE Green, KJ Menzies, JD Crane
    Molecular Metabolism, 2022-08-17;65(0):101575.
    Species: Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair
    Authors: C Blessing, K Apelt, D van den He, C Gonzalez-L, MB Rother, M van der Wo, R González-P, A Yifrach, A Parnas, RG Shah, TT Kuo, DEC Boer, J Cai, A Kragten, HS Kim, OD Schärer, ACO Vertegaal, GM Shah, S Adar, H Lans, H van Attiku, AG Ladurner, MS Luijsterbu
    Nature Communications, 2022-08-13;13(1):4762.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Development of Olaparib-Resistance Prostate Cancer Cell Lines to Identify Mechanisms Associated with Acquired Resistance
    Authors: M Cahuzac, B Péant, AM Mes-Masson, F Saad
    Cancers, 2022-08-11;14(16):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. BTApep-TAT peptide inhibits ADP-ribosylation of BORIS to induce DNA damage in cancer
    Authors: Y Zhang, M Fang, S Li, H Xu, J Ren, L Tu, B Zuo, W Yao, G Liang
    Molecular Cancer, 2022-08-02;21(1):158.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Co-Immunoprecipitation
  24. Compartmentalized regulation of NAD+ by Di (2-ethyl-hexyl) phthalate induces DNA damage in placental trophoblast
    Authors: S Zhao, Y Hong, YY Liang, XL Li, JC Shen, CC Sun, LL Chu, J Hu, H Wang, DX Xu, SC Zhang, DD Xu, T Xu, LL Zhao
    Redox Biology, 2022-07-20;55(0):102414.
    Species: Human, Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Deubiquitinating enzymes and the proteasome regulate preferential sets of ubiquitin substrates
    Authors: F Trulsson, V Akimov, M Robu, N van Overbe, DAP Berrocal, RG Shah, J Cox, GM Shah, B Blagoev, ACO Vertegaal
    Nature Communications, 2022-05-18;13(1):2736.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  26. Tankyrase-mediated ADP-ribosylation is a regulator of TNF-induced death
    Authors: L Liu, JJ Sandow, DM Leslie Ped, AL Samson, N Silke, T Kratina, RL Ambrose, M Doerflinge, Z Hu, E Morrish, D Chau, AJ Kueh, C Fitzibbon, M Pellegrini, JS Pearson, MO Hottiger, AI Webb, N Lalaoui, J Silke
    Science Advances, 2022-05-11;8(19):eabh2332.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  27. The lactate-NAD+ axis activates cancer-associated fibroblasts by downregulating p62
    Authors: JF Linares, T Cid-Diaz, A Duran, M Osrodek, A Martinez-O, M Reina-Camp, HH Kuo, O Elemento, ML Martin, T Cordes, TC Thompson, CM Metallo, J Moscat, MT Diaz-Meco
    Cell Reports, 2022-05-10;39(6):110792.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  28. PARP inhibitors trap PARP2 and alter the mode of recruitment of PARP2 at DNA damage sites
    Authors: Xiaohui Lin, Wenxia Jiang, Johannes Rudolph, Brian J Lee, Karolin Luger, Shan Zha
    Nucleic Acids Research
  29. Pre-activation of autophagy impacts response to olaparib in prostate cancer cells
    Authors: Maxime Cahuzac, Patricia Langlois, Benjamin Péant, Hubert Fleury, Anne-Marie Mes-Masson, Fred Saad
    Communications Biology
  30. Protective Effect of Minocycline Hydrochloride on the Mouse Embryonic Development Against Suboptimal Environment
    Authors: Xiaojing Hou, Changming Cai, Yuanlin He, Shiyu An, Shuqin Zhao, Hao Sun et al.
    Frontiers in Cell and Developmental Biology
  31. Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis
    Authors: Q Chen, K Ma, X Liu, SH Chen, P Li, Y Yu, AKL Leung, X Yu
    Cell Discovery, 2022-01-18;8(1):3.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  32. PARP inhibitor BMN-673 induced apoptosis by trapping PARP-1 and inhibiting base excision repair via modulation of pol-beta in chromatin of breast cancer cells
    Authors: C Sethy, CN Kundu
    Toxicology and Applied Pharmacology, 2022-01-05;436(0):115860.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  33. Interaction of DBC1 with polyoma small T antigen promotes its degradation and negatively regulates tumorigenesis
    Authors: Z Sarwar, N Nabi, SA Bhat, SQ Gillani, I Reshi, M Un Nisa, G Adelmant, J Marto, S Andrabi
    The Journal of Biological Chemistry, 2021-12-16;0(0):101496.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, ICC, Immunoprecipitation, Western Blot
  34. Timely termination of repair DNA synthesis by ATAD5 is important in oxidative DNA damage-induced single-strand break repair
    Authors: SH Park, Y Kim, JS Ra, MW Wie, MS Kang, S Kang, K Myung, KY Lee
    Nucleic Acids Research, 2021-11-18;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  35. Contribution of NADPH oxidase to the retention of UVR-induced DNA damage by arsenic
    Authors: KL Cooper, LB Volk, DR Dominguez, AD Duran, KJ Ke Jian Li, LG Hudson
    Toxicology and Applied Pharmacology, 2021-11-16;434(0):115799.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Capture
  36. TARG1 protects against toxic DNA ADP-ribosylation
    Authors: C Tromans-Co, A Sanchi, GK Moeller, G Timinszky, M Lopes, I Ahel
    Nucleic Acids Research, 2021-10-11;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. PARylation prevents the proteasomal degradation of topoisomerase I DNA-protein crosslinks and induces their deubiquitylation
    Authors: Y Sun, J Chen, SN Huang, YP Su, W Wang, K Agama, S Saha, LM Jenkins, JM Pascal, Y Pommier
    Nature Communications, 2021-08-18;12(1):5010.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  38. PARP1 and CHK1 coordinate PLK1 enzymatic activity during the DNA damage response to promote homologous recombination-mediated repair
    Authors: B Peng, R Shi, J Bian, Y Li, P Wang, H Wang, J Liao, WG Zhu, X Xu
    Nucleic Acids Research, 2021-07-21;49(13):7554-7570.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Immunoprecipitation
  39. MTH1 and OGG1 maintain a low level of 8-oxoguanine in Alzheimer's brain, and prevent the progression of Alzheimer's pathogenesis
    Authors: S Oka, J Leon, K Sakumi, N Abolhassan, Z Sheng, D Tsuchimoto, FM LaFerla, Y Nakabeppu
    Scientific Reports, 2021-03-23;11(1):5819.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  40. Selective modulation by PARP-1 of HIF-1&alpha-recruitment to chromatin during hypoxia is required for tumor adaptation to hypoxic conditions
    Authors: JM Martí, A Garcia-Dia, D Delgado-Be, F O'Valle, A González-F, O Carlevaris, JM Rodríguez-, JC Amé, F Dantzer, GL King, K Dziedzic, E Berra, E de Álava, AT Amaral, EM Hammond, FJ Oliver
    Redox Biology, 2021-02-01;41(0):101885.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  41. Kaposi's sarcoma-associated herpesvirus processivity factor (PF-8) recruits cellular E3 ubiquitin ligase CHFR to promote PARP1 degradation and lytic replication
    Authors: WC Chung, S Lee, Y Kim, JB Seo, MJ Song
    PloS Pathogens, 2021-01-28;17(1):e1009261.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  42. Clinical PARP inhibitors do not abrogate PARP1 exchange at DNA damage sites in vivo
    Authors: Z Shao, BJ Lee, É Rouleau-Tu, MF Langelier, X Lin, VM Estes, JM Pascal, S Zha
    Nucleic Acids Res., 2020-09-25;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  43. Poly(ADP-ribose) Polymerase 1 (PARP1) restrains MyoD-dependent gene expression during muscle differentiation
    Authors: F Matteini, O Andresini, S Petrai, C Battistell, MN Rossi, R Maione
    Sci Rep, 2020-09-15;10(1):15086.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  44. Inhibition of nicotinamide phosphoribosyltransferase (NAMPT) with OT-82 induces DNA damage, cell death, and suppression of tumor growth in preclinical models of Ewing sarcoma
    Authors: AE Gibson, C Yeung, SH Issaq, VJ Collins, M Gouzoulis, Y Zhang, J Ji, A Mendoza, CM Heske
    Oncogenesis, 2020-09-10;9(9):80.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Functional Assay
  45. XRN2 interactome reveals its synthetic lethal relationship with PARP1 inhibition
    Authors: PL Patidar, T Viera, JC Morales, N Singh, EA Motea, M Khandelwal, FJ Fattah
    Sci Rep, 2020-08-28;10(1):14253.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  46. Pathogenic ARH3 mutations result in ADP-ribose chromatin scars during DNA strand break repair
    Authors: H Hanzlikova, E Prokhorova, K Krejcikova, Z Cihlarova, I Kalasova, J Kubovciak, J Sachova, R Hailstone, J Brazina, S Ghosh, S Cirak, JG Gleeson, I Ahel, KW Caldecott
    Nat Commun, 2020-07-07;11(1):3391.
  47. Poly (ADP-ribose) polymerase 1 inhibition prevents neurodegeneration and promotes alpha-synuclein degradation via transcription factor EB-dependent autophagy in mutant alpha-synucleinA53T model of Parkinson's disease
    Authors: K Mao, J Chen, H Yu, H Li, Y Ren, X Wu, Y Wen, F Zou, W Li
    Aging Cell, 2020-05-31;0(0):e13163.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. Crosstalk between hydroxytyrosol, a major olive oil phenol, and HIF-1 in MCF-7 breast cancer cells
    Authors: J Calahorra, E Martínez-L, JM Granadino-, JM Martí, A Cañuelo, S Blanco, FJ Oliver, E Siles
    Sci Rep, 2020-04-14;10(1):6361.
    Species: Human
    Sample Types: CCS MCF-7 cell line
  49. Poly(ADP-ribosyl)ation mediates early phase histone eviction at DNA lesions
    Authors: G Yang, Y Chen, J Wu, SH Chen, X Liu, AK Singh, X Yu
    Nucleic Acids Res., 2020-04-06;0(0):.
    Species: Human
    Sample Types: Cell Lysate
    Applications: Immunoprecipitation
  50. Tankyrase disrupts metabolic homeostasis and promotes tumorigenesis by inhibiting LKB1-AMPK signalling
    Authors: N Li, Y Wang, S Neri, Y Zhen, LWR Fong, Y Qiao, X Li, Z Chen, C Stephan, W Deng, R Ye, W Jiang, S Zhang, Y Yu, MC Hung, J Chen, SH Lin
    Nat Commun, 2019-09-25;10(1):4363.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  51. MORC2 regulates DNA damage response through a PARP1-dependent pathway
    Authors: L Zhang, DQ Li
    Nucleic Acids Res., 2019-09-19;47(16):8502-8520.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  52. A PARP1-BRG1-SIRT1 axis promotes HR repair by reducing nucleosome density at DNA damage sites
    Authors: Y Chen, H Zhang, Z Xu, H Tang, A Geng, B Cai, T Su, J Shi, C Jiang, X Tian, A Seluanov, J Huang, X Wan, Y Jiang, V Gorbunova, Z Mao
    Nucleic Acids Res., 2019-09-19;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  53. Poly (ADP) ribose glycohydrolase can be effectively targeted in pancreatic cancer
    Authors: A Jain, LC Agostini, GA McCarthy, SN Chand, A Ramirez, A Nevler, J Cozzitorto, CW Schultz, CY Lowder, KM Smith, ID Waddell, M Raitses-Gu, C Stossel, YG Gorman, D Atias, CJ Yeo, JM Winter, KP Olive, T Golan, MJ Pishvaian, D Ogilvie, DI James, AM Jordan, JR Brody
    Cancer Res., 2019-07-04;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  54. Acquired Resistance of EGFR-Mutated Lung Cancer to Tyrosine Kinase Inhibitor Treatment Promotes PARP Inhibitor Sensitivity
    Authors: L Marcar, K Bardhan, L Gheorghiu, P Dinkelborg, H Pfäffle, Q Liu, M Wang, Z Piotrowska, LV Sequist, K Borgmann, JE Settleman, JA Engelman, AN Hata, H Willers
    Cell Rep, 2019-06-18;27(12):3422-3432.e4.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  55. The coronavirus macrodomain is required to prevent PARP-mediated inhibition of virus replication and enhancement of IFN expression
    Authors: ME Grunewald, Y Chen, C Kuny, T Maejima, R Lease, D Ferraris, M Aikawa, CS Sullivan, S Perlman, AR Fehr
    PLoS Pathog., 2019-05-16;15(5):e1007756.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  56. Overexpressed ABCB1 Induces Olaparib-Taxane Cross-Resistance in Advanced Prostate Cancer
    Authors: AP Lombard, C Liu, CM Armstrong, LS D'Abronzo, W Lou, H Chen, M Dall'Era, PM Ghosh, CP Evans, AC Gao
    Transl Oncol, 2019-05-07;12(7):871-878.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  57. PARP-1 Is Critical for Recruitment of Dendritic Cells to the Lung in a Mouse Model of Asthma but Dispensable for Their Differentiation and Function
    Authors: LC Echeverri, MA Ghonim, J Wang, AA Al-Khami, D Wyczechows, HH Luu, H Kim, MD Sanchez-Pi, J Yélamos, LM Yassin, AH Boulares
    Mediators Inflamm., 2019-04-24;2019(0):1656484.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  58. Poly(ADP-ribose) polymerase 1 accelerates vascular calcification by upregulating Runx2
    Authors: C Wang, W Xu, J An, M Liang, Y Li, F Zhang, Q Tong, K Huang
    Nat Commun, 2019-03-13;10(1):1203.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  59. NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression
    Authors: C Bian, C Zhang, T Luo, A Vyas, SH Chen, C Liu, MA Kassab, Y Yang, M Kong, X Yu
    Nat Commun, 2019-02-11;10(1):693.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  60. Mono-ADP-ribosylation of H3R117 traps 5mC hydroxylase TET1 to impair demethylation of tumor suppressor gene TFPI2
    Authors: M Li, Y Tang, Q Li, M Xiao, Y Yang, Y Wang
    Oncogene, 2019-01-16;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  61. Tripartite Motif-containing 33 (TRIM33) protein functions in the poly(ADP-ribose) polymerase (PARP)-dependent DNA damage response through interaction with Amplified in Liver Cancer 1 (ALC1) protein.
    Authors: Kulkarni A, Oza J, Yao M, Sohail H, Ginjala V, Tomas-Loba A, Horejsi Z, Tan A, Boulton S, Ganesan S
    J Biol Chem, 2013-08-06;288(45):32357-69.
  62. The PARP3- and ATM-dependent phosphorylation of APLF facilitates DNA double-strand break repair.
    Authors: Fenton A, Shirodkar P, Macrae C, Meng L, Koch C
    Nucleic Acids Res, 2013-02-28;41(7):4080-92.
  63. BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8.
    Authors: Yan Q, Xu R, Zhu L, Cheng X, Wang Z, Manis J, Shipp M
    Mol Cell Biol, 2012-12-10;33(4):845-57.
  64. Loss of 53BP1 causes PARP inhibitor resistance in Brca1-mutated mouse mammary tumors.
    Authors: Jaspers J, Kersbergen A, Boon U, Sol W, van Deemter L, Zander S, Drost R, Wientjens E, Ji J, Aly A, Doroshow J, Cranston A, Martin N, Lau A, O'Connor M, Ganesan S, Borst P, Jonkers J, Rottenberg S
    Cancer Discov, 2012-10-25;3(1):68-81.

FAQs

  1. What is the concentration of PAR/pADPr Antibody (Catalog # 4335-MC-100)

    • The PAR/pADPr Antibody (Catalog # 4335-MC-100) is supplied as a solution in TBS containing glycerol, at a concentration of 100 µg/100 µL.

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PAR/pADPr Antibody
By Anonymous on 03/15/2024
Application: WB Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

SDS-PAGE 10% gel of THP-1 monocytes with/without 5 mins 2mM H2O2 treatment. 30ug of protein run. Anti-PAR/pADPr used at 1:2,000 diln in 5% BSA in TBS-T for 1h.

Western Blot PAR/pADPr Antibody 4335-MC-100
PAR/pADPr Antibody
By Anonymous on 03/08/2024
Application: IP Sample Tested: THP-1 human acute monocytic leukemia cell line Species: Human

Antibody was used to perform immunoprecipitation (2ug) of THP-1 lysates before SDS-PAFE of samples and immunoblot was performed with anti-CTCF (abcam ab188408 1:5k diln). Parylated-CTCF found at 180kDa in righthand lane.

Immunoprecipitation PAR/pADPr Antibody 4335-MC-100
PAR/pADPr Antibody
By Marina Rodriguez on 02/02/2021
Application: WB Sample Tested: Breast cancer cells Species: Human

8% polyacrylamide gel run until 55 kDa band. 1:750 primary antibody dilution; 1:10000 secondary antibody dilution. UT=untreated cells.