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Phalloidin-TRITC

Catalog #: 5783 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
5783/12U
Phalloidin-TRITC | CAS No. 915013-10-4 | Fluorescent Actin Probes
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Description: Red-orange fluorescent cytoskeleton stain. Binds and labels F-actin. Used for: F-actin staining. Application: fluorescent microscopy, immunofluorescence (IHC, ICC)

Chemical Name: 7-[(4R)-5-[[[[4-[3,6-Bis(dimethylamino)xanthylium-9-yl]-3-carboxyphenyl]amino]thioxomethyl]amino]-4-hydroxy-L-leucine]phalloidin

Purity: ≥90%

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Biological Activity

Key information: Phalloidin-TRITC is a red-orange fluorescent cytoskeleton stain. It binds and labels specifically F-actin.

Used for: Phalloidin-TRITC can be used to visualize and quantify F-actin in tissue sections, cell cultures or cell-free experiments with optimal results in formaldehyde-fixed and permeabilized cells.

Application: fluorescent microscopy, immunofluorescence (IHC, ICC).

Properties and Photophysical Data: Phalloidin-TRITC is composed of the F-actin probe, Phalloidin (Cat. No. 4535), conjugated to Tetramethylrhodamine isothiocyanate (TRITC) dye. Phalloidin binds F-actin with high selectivity allowing Phalloidin-TRITC to stain actin with high-contrast discrimination. Excitation and emission maxima (λ) are 540 nm and 565 nm, respectively.

We suggest making up a stock solution of this product by dissolving the vial contents in 1.5 mL methanol or DMSO. Please refer to the protocol for further information on using this product.

Scientific Data

Fluorescence Imaging Applications in HeLa Cells for Phalloidin-TRITC View Larger

Applications in HeLa Cells for Phalloidin-TRITC. Application of Phalloidin-TRITC: HeLa cells were labeled with 150 nM Phalloidin-TRITC for 20 minutes. Counterstained with DAPI (Catalog # 5748).

Technical Data

M.Wt:
1231.4
Formula:
C60H70N12O13S2
Purity:
≥90%
Storage:
Store at -20°C
CAS No:
915013-10-4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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