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Phospho-MARCKS (S152/S156) Antibody

Catalog #: PPS010
2 Citations Datasheet / COA / SDS
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PPS010
Detection of Phospho-MARCKS (S152/S156) by Western Blot.
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Citations (2)
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Phospho-MARCKS (S152/S156) Antibody Summary

Species Reactivity
Human, Mouse, Rat, Bovine, Chicken, Xenopus, Zebrafish
Specificity
Specific for the ~83 kDa MARCKS protein phosphorylated at S152/S156 in Western blots of rat brain homogenates.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-S152/S156 of MARCKS
Formulation
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Phospho-MARCKS (S152/S156) antibody by Western Blot. View Larger

Detection of Phospho-MARCKS (S152/S156) by Western Blot. Western blot of rat brain lysate showing specific immunolabeling of the ~83 kDa MARCKS protein phosphorylated at S152/S156 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-MARCKS (S152/S156). The immunolabeling is completely eliminated by treatment with lambda -PPase.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: MARCKS

Rat MARCKS (Myristoylated, Alanine-rich C Kinase protein Substrate) is an 83 kDa, 309 amino acid intracellular protein that belongs to the MARCKS family of molecules. It is an actin-organizing molecule and a key substrate for activated protein kinase C (PKC) and PKC-related kinase. MARCKS is normally sequestered on the inner face of the plasma membrane. Here, it is bound to phosphatidylserine (PS) via electrostatic and hydrophobic interactions. Upon integrin ligation ( alpha 5 beta 1), PKC is activated, and phosphorylates MARCKS at S152 and S156. This breaks the PS interaction, forcing MARCKS into the cytosol. Subsequent cytosolic dephosphorylation of MARCKS induces MARCKS-mediated polymerization of actin and its return to the membrane.

Long Name
Myristoylated Alanine-rich Protein Kinase C Substrate
Entrez Gene IDs
4082 (Human); 17118 (Mouse); 25603 (Rat)
Alternate Names
80K-L protein; 80K-L; FLJ14368; MACS; MACSmyristoylated alanine-rich C-kinase substrate; MARCKS; MRACKS; myristoylated alanine-rich protein kinase C substrate (MARCKS, 80K-L); myristoylated alanine-rich protein kinase C substrate; phosphomyristin; PKCSLFLJ90045; PRKCSL; Protein kinase C substrate, 80 kDa protein, light chain

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Citations for Phospho-MARCKS (S152/S156) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cardiomyocyte stromal interaction molecule 1 is a key regulator of Ca2+ -dependent kinase and phosphatase activity in the mouse heart
    Authors: HE Collins, JC Anderson, AR Wende, JC Chatham
    Physiological Reports, 2022-02-01;10(4):e15177.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release.
    Authors: Obis T, Besalduch N, Hurtado E, Nadal L, Santafe M, Garcia N, Tomas M, Priego M, Lanuza M, Tomas J
    Mol Brain, 2015-02-10;8(0):8.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Western Blot

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