Phospho-Tyrosine Antibody

Name: Phospho-Tyrosine Antibody
Brand: R&D Systems
SKU: MAB16761
Rating: 5 (1 reviews)

Catalog #: MAB16761
1 Citation 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB16761
MAB16761-SP

Detection of Phospho-Tyrosine by Western Blot.

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All Phospho-Tyrosine Antibodies

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Phospho-Tyrosine Antibody Summary

Specificity

Detects proteins containing phosphorylated tyrosine residues in Western blots. In Western blots of pervanadate-treated cell lysates, clone 216954 binds Phospho-Tyrosine in a broad manner largely independent of the surrounding amino acid sequence. No cross-reactivity with proteins or peptides containing phosphorylated serine or threonine residues is observed.

Source

Monoclonal Rat IgG_2A Clone # 216954

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

KLH-coupled Phospho-Tyrosine synthetic peptide

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

8-25 µg/mL

Immersion fixed A431 human epithelial carcinoma cell line treated with pervanadate

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Phospho-Tyrosine by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 50 µM pervanadate (PV) for 15 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Phospho-Tyrosine Monoclonal Antibody (Catalog # MAB16761), followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Tyrosine-phosphorylated proteins were detected (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Phospho-Tyrosine

Phosphorylation of tyrosine residues in signaling proteins by protein tyrosine kinases mediates a variety of cellular processes, including cell growth, differentiation, adhesion, motility, death, and metabolism. Dysregulation of tyrosine phosphorylation has been implicated in the development of many human diseases, such as diabetes and cancer. Antibodies specific for phospho-tyrosine have been invaluable reagents in the studies of signaling pathways initiated by tyrosine phosphorylation.

Alternate Names

2 amino 3(4 hydroxyphenyl) propanoic acid; 4 hydroxyphenylalanine; PhosphoTyrosine; pTyrosine; pY20; Tyrosine

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

GDNF Family

Citation for Phospho-Tyrosine Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Developmental and cell cycle regulation of the Drosophila histone locus body.
Authors: White AE, Leslie ME, Calvi BR, Marzluff WF, Duronio RJ
Mol. Biol. Cell, 2007-04-18;18(7):2491-502.
Species: Drosophila
Sample Types: Whole Tissue
Applications: IHC-Fr