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Porcine IL-10 Antibody

Catalog #: MAB6931
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6931
MAB6931-SP
Cell Proliferation Induced by IL‑10 and Neutralization by Porcine IL‑10 Antibody.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Porcine IL-10 Antibody Summary

Species Reactivity
Porcine
Specificity
Detects porcine IL‑10 in direct ELISAs. In direct ELISAs, 25-50% cross-reactivity with recombinant feline IL-10, recombinant canine IL-10, and recombinant guinea pig IL-10 is observed, approximately 10% cross-reactivity with recombinant human IL-10 is observed, less than 5% cross-reactivity with recombinant cotton rat IL-10 is observed, and no cross-reactivity with recombinant equine IL-10, recombinant mouse IL-10, recombinant rat IL-10, or recombinant viral IL-10 is observed.
Source
Monoclonal Mouse IgG2B Clone # 148801
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant porcine IL-10
Ser19-Asn175
Accession # Q29055
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 40 ng/mL Recombinant Porcine IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced by IL‑10 and Neutralization by Porcine IL‑10 Antibody. View Larger

Cell Proliferation Induced by IL‑10 and Neutralization by Porcine IL‑10 Antibody. Recombinant Porcine IL-10 (Catalog # 693-PI) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-10 Mono-clonal Antibody (Catalog # MAB6931). The ND50 is typically 0.05-0.15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of murine T helper 2 (Th2) clones that inhibited the cytokine production by Th1 clones which are dependent upon stimulation with antigen in the presence of antigen presenting cells (APC). Murine IL-10 is produced by Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, and LY-1+ (CD5+) and normal B cells. Human IL-10 has cross-species actvities and is active on mouse cells. Murine IL-10 is species-specific and does not act on human cells. Porcine IL-10 shares 71% and 78% amino acid sequence identitywith mouse and human IL-10, respectively. IL-10 is a pleiotropic cytokine that can exert either immunostimulatory or immunosuppressive effects on a variety of cell types. It is a potent immunosuppressant of macrophage functions. In vitro, IL-10 can inhibit the accessory function and antigen-presenting capacity of monocytes by, among other effects, down‑regulating class II MHC expression. Thus, IL-10 can inhibit monocyte/macrophage-dependent, antigen stimulated cytokine synthesis (especially IFN-gamma ) by human PBMNC and NK, and mouse Th1 cells. Additionally, IL-10 is a potent inhibitor of monocyte/macrophage activation and its resultant cytotoxic effects. As an immunostimulatory cytokine, IL-10 can act on B cells to enhance their viability, cell proliferation, Ig secretion, and class II MHC expression. Aside from B lymphocytes, IL-10 is also a growth co-stimulator for thymocytes and mast cells, as well as an enhancer of cytotoxic T cell development.

References
  1. Moore, K.W. et al. (1993) Annu. Rev. Immunol. 11:165.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Citations for Porcine IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Food Contaminant Zearalenone and Its Metabolites Affect Cytokine Synthesis and Intestinal Epithelial Integrity of Porcine Cells
    Authors: Daniela E. Marin, Monica Motiu, Ionelia Taranu
    Toxins (Basel)
  2. Analysis of Porcine Pro- and Anti-Inflammatory Cytokine Induction by S. suis In Vivo and In Vitro
    Authors: FS Hohnstein, M Meurer, N de Buhr, M von Köckri, CG Baums, G Alber, N Schütze
    Pathogens, 2020-01-03;9(1):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Postnatal persistent infection with classical Swine Fever virus and its immunological implications.
    Authors: Munoz-Gonzalez S, Ruggli N, Rosell R, Perez L, Frias-Leuporeau M, Fraile L, Montoya M, Cordoba L, Domingo M, Ehrensperger F, Summerfield A, Ganges L
    PLoS ONE, 2015-05-04;10(5):e0125692.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Neutralization
  4. The effect of activated protein C on plasma cytokine levels in a porcine model of acute endotoxemia.
    Authors: Nielsen JS, Larsson A, Rix T, Nyboe R, Gjedsted J, Krog J, Ledet T, Tonnesen E
    Intensive Care Med, 2007-04-25;33(6):1085-93.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  5. beta -Glucan-Induced IL-10 Secretion by Monocytes Triggers Porcine NK Cell Cytotoxicity
    Authors: Leen Hermans, Steffi De Pelsmaeker, Sofie Denaeghel, Eric Cox, Herman W. Favoreel, Bert Devriendt
    Frontiers in Immunology

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