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Porcine IL-10 ELISA Kit - Quantikine

Catalog #: P1000
19 Citations Datasheet / COA / SDS
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P1000
Porcine IL-10 ELISA Standard Curve
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Product Details
Procedure
Citations (19)
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Summary Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Porcine IL-10 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
Sensitivity
5.5 pg/mL
Assay Range
31.3 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant porcine IL-10
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Porcine IL-10 Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-10 levels in cell culture supernates, porcine serum, and EDTA plasma. It contains E. coli-expressed recombinant porcine IL-10 and antibodies raised against the recombinant protein. Results obtained for naturally occurring porcine IL-10 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural porcine IL-10.

Recovery

The recovery of porcine IL-10 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=4) 106 101-109
EDTA Plasma (n=4) 92 80-100
Serum (n=4) 94 84-102

Linearity

To assess the linearity of the assay, four samples spiked with high concentrations of porcine IL-10 in each matrix were diluted with Calibrator Diluent and then assayed.
Porcine IL-10 ELISA Linearity

Scientific Data

N/A Porcine IL-10 ELISA Standard Curve View Larger

Porcine IL-10 ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-10

Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.

Long Name:
Interleukin 10
Entrez Gene IDs:
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names:
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 5 times.
    8. 120 µL Substrate Solution
  8.   Add 120 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 120 µL Stop Solution
  9.   Add 120 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Porcine IL-10 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. Vitamin 25(OH)D3, E, and C Supplementation Impact the Inflammatory and Antioxidant Responses in Piglets Fed a Deoxynivalenol-Contaminated Diet and Challenged with Lipopolysaccharides
    Authors: Sauvé, B;Chorfi, Y;Létourneau-Montminy, MP;Guay, F;
    Toxins
    Species: Porcine
    Sample Types: Plasma
  2. Regenerative Effect of Mesenchymal Stem Cell on Cartilage Damage in a Porcine Model
    Authors: Lin, S;Panthi, S;Hsuuw, Y;Chen, S;Huang, M;Sieber, M;Hsuuw, Y;
    Biomedicines
    Species: Porcine
    Sample Types: Serum
  3. Using a concurrent challenge with porcine circovirus 2 and porcine reproductive and respiratory syndrome virus to compare swine vaccination programs
    Authors: A Madapong, K Saeng-Chut, A Tantituvan, D Nilubol
    Scientific Reports, 2022-09-15;12(1):15524.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  4. Intranasal delivery of inactivated PRRSV loaded cationic nanoparticles coupled with enterotoxin subunit B induces PRRSV-specific immune responses in pigs
    Authors: P Chaikhumwa, A Madapong, K Saeng-Chut, D Nilubol, A Tantituvan
    Scientific Reports, 2022-03-08;12(1):3725.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  5. Overexpression of Substance P in pig airways increases MUC5AC through an NF-k&beta pathway
    Authors: M Sponchiado, YS Liao, KR Atanasova, EN Collins, V Schurmann, L Bravo, LR Reznikov
    Physiological Reports, 2021-02-01;9(3):e14749.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  6. Protective effects of new femoral reaming techniques (Reamer irrigator aspirator, RIA I and II) on pulmonary function and posttraumatic contusion (CT morphology) - results from a standardized large animal model
    Authors: S Halvachiza, M Teuben, M Lempert, Y Kalbas, N Cesarovic, M Lipiski, E Benninger, P Cinelli, R Pfeifer, HC Pape
    Injury, 2020-10-06;0(0):.
    Species: Porcine
    Sample Types: BALF
  7. Immuno-modulating properties of Tulathromycin in porcine monocyte-derived macrophages infected with porcine reproductive and respiratory syndrome virus
    Authors: D Desmonts d, R Moges, A Siddiq, T Allain, TD Feener, GP Muench, N McKenna, RM Yates, AG Buret
    PLoS ONE, 2019-08-23;14(8):e0221560.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  8. Influence of zinc supplementation on immune parameters in weaned pigs
    Authors: V Kloubert, K Blaabjerg, TS Dalgaard, HD Poulsen, L Rink, I Wessels
    J Trace Elem Med Biol, 2018-01-31;0(0):.
    Species: Porcine
    Sample Types: Whole Blood
    Applications: ELISA Capture
  9. Beneficial effects of inhaled nitric oxide with intravenous steroid in an ischemia-reperfusion model involving aortic clamping
    Authors: W Gozdzik, S Zielinski, M Zielinska, K Ratajczak, P Skrzypczak, S Rodziewicz, A Kübler, K Löfström, P Dziegiel, M Olbromski, B Adamik, S Ryniak, P Harbut, J Albert, C Frostell
    Int J Immunopathol Pharmacol, 2018-01-01;32(0):3946320177514.
    Species: Porcine
    Sample Types: Plasma
  10. Butyrate modifies intestinal barrier function in IPEC-J2 cells through a selective upregulation of tight junction proteins and activation of the Akt signaling pathway
    Authors: H Yan, KM Ajuwon
    PLoS ONE, 2017-06-27;12(6):e0179586.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  11. Effect of Lactobacillus salivarius bacteriocin Abp118 on the mouse and pig intestinal microbiota.
    Authors: Riboulet-Bisson E, Sturme MH, Jeffery IB
    PLoS ONE, 2012-02-17;7(2):e31113.
    Species: Porcine
    Sample Types: Serum
  12. Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus.
    Authors: Jeong HJ, Song YJ, Lee SW
    Clin. Vaccine Immunol., 2010-02-03;17(4):503-12.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  13. Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues.
    Authors: Wall R, Ross RP, Shanahan F, O'Mahony L, O'Mahony C, Coakley M, Hart O, Lawlor P, Quigley EM, Kiely B, Fitzgerald GF, Stanton C
    Am. J. Clin. Nutr., 2009-04-08;89(5):1393-401.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  14. Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
    Authors: Thorgersen EB, Pharo A, Haverson K, Axelsen AK, Gaustad P, Kotwal GJ, Sfyroera G, Mollnes TE
    Infect. Immun., 2008-12-01;77(2):725-32.
    Species: Porcine
    Sample Types: Whole Blood
  15. Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.
    Authors: Kruse R, Essen-Gustavsson B, Fossum C, Jensen-Waern M
    Acta Vet. Scand., 2008-08-12;50(0):32.
    Species: Porcine
    Sample Types: Serum
  16. Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
    Authors: Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE
    J. Immunol., 2008-05-01;180(9):6346-53.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  17. Experimental severe Pseudomonas aeruginosa pneumonia and antibiotic therapy in piglets receiving mechanical ventilation.
    Authors: Luna CM, Baquero S, Gando S, Patron JR, Morato JG, Sibila O, Absi R, Famiglietti A, Vay CA, Von Stecher F, Agusti C, Torres A
    Chest, 2007-08-01;132(2):523-31.
    Species: Porcine
    Sample Types: Serum
  18. Butyrate differentially regulates cytokines and proliferation in porcine peripheral blood mononuclear cells.
    Authors: Weber TE, Kerr BJ
    Vet. Immunol. Immunopathol., 2006-05-24;113(1):139-47.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  19. Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide.
    Authors: Changhua L, Jindong Y, Defa L, Lidan Z, Shiyan Q, Jianjun X
    J. Nutr., 2005-02-01;135(2):239-44.
    Species: Porcine
    Sample Types: Serum

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