Porcine IL-18/IL-1F4 Biotinylated Antibody

Catalog #: BAF588 Datasheet / COA / SDS

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BAF588

All IL-18/IL-1F4 Antibodies

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Porcine IL-18/IL-1F4 Biotinylated Antibody Summary

Species Reactivity

Porcine

Specificity

Detects porcine IL‑18/IL‑1F4 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human IL-18 is observed and less than 1% cross-reactivity with recombinant mouse IL-18 and recombinant rat IL-18 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant porcine IL‑18/IL‑1F4

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Porcine IL‑18/IL‑1F4 (Catalog # 588-PL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-18/IL-1F4

Interleukin-18 (IL-18), also known as IL-1F4 and IFN-gamma inducing factor (IGIF), is a member of the IL-1 family of cytokines and is a key molecule in the innate immune response (1). Porcine IL-18 is synthesized as a 24 kDa proprotein that contains a 35 amino acid (aa) propeptide and a 157 aa mature region (2). Under inflammatory conditions, the propeptide is cleaved by Caspase-1 in the cytoplasm to liberate the mature nonglycosylated 18 kDa monomeric IL-18 (3, 4). Mature porcine IL-18 shares 88% aa sequence identity with canine and feline IL-18 and 61%‑76% with human, mouse, rat, and rhesus IL-18. IL-18 is secreted by a variety of cell types including macrophages, dendritic cells, and epithelial cells (1, 5). Circulating mature IL-18 is sequestered by soluble IL-18 binding proteins (IL-18 BP) that inhibit IL-18 bioactivity (6). IL-18 interacts with the widely expressed IL-18 R alpha which then recruits the signaling subunit IL-18 R beta (7, 8). The IL-1 family member IL-1F7 also binds to IL-18 R alpha but does not recruit IL-18 R beta or induce signaling (9). IL-1F7 binds IL-18 BP and enhances its neutralizing effect on IL-18 activity (9). IL-18 synergizes with other cytokines to activate NK, Th1, and Th17 cells and to increase the production of IFN-gamma (1, 5, 10, 11, 12). IL-18 can also promote Th2 cytokine release which reduces the effectiveness of antiviral responses (13, 14). Increased levels of active IL‑18 contribute to the severity of autoimmunity and hypertension, while deficiency of IL-18 results in symptoms of metabolic syndrome (1, 5, 15, 16). In cancer, IL-18 stimulates Th1 and NK cells to target tumor cells, but it can also promote angiogenesis, metastasis, and tumor cell immune evasion (11).

References

Arend, W.P. et al. (2008) Immunol. Rev. 223:20.
Muneta, Y. et al. (2000) Cytokine 12:566.
Ghayur, T. et al. (1997) Nature 386:619.
Gu, Y. et al. (1997) Science 275:206.
Boraschi, D. and C.A. Dinarello (2006) Eur. Cytokine Netw. 17:224.
Novick, D. et al. (1999) Immunity 10:127.
Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Bufler, P. et al. (2002) Proc. Natl. Acad. Sci. 99:13723.
Takeda, K. et al. (1998) Immunity 8:383.
Park, S. et al. (2007) Cell. Mol. Immunol. 4:329.
Yoshimoto, T. et al. (1998) J. Immunol. 161:3400.
Hoshino, T. et al. (2001) J. Immunol. 166:7014.
Iannello, A. et al. (2009) AIDS Rev. 11:115.
Rabkin, S.W. (2009) Nat. Clin. Pract. Cardiovasc. Med. 6:192.
Netea, M.G. et al. (2006) Nat. Med. 12:650.

Long Name

Interleukin 18

Entrez Gene IDs

3606 (Human); 16173 (Mouse); 29197 (Rat); 397057 (Porcine); 574151 (Primate)

Alternate Names

Iboctadekin; IFN-gamma-inducing factor; IGIF; IGIFIL-1 gamma; IL18; IL-18; IL-18MGC12320; IL-1F4; IL1F4iboctadekin; IL-1g; Interferon gamma-inducing factor; interleukin 18 (interferon-gamma-inducing factor); Interleukin-1 gamma; interleukin-18