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Porcine PDGF Protein, CF

Catalog #: 125-PD
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
125-PD-005
Product Details
Citations (3)
FAQs
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Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Porcine PDGF Protein, CF Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.

The ED50 for this effect is 6-60 ng/mL in a fluorometric assay using the redox sensitive dye, Resazurin (Catalog # AR002)  and 1-3 ng/mL when measured by 3H-thymidine incorporation.

Source
Porcine platelet-derived PDGF protein
SDS-PAGE
18-28 kDa, reducing conditions

Product Datasheets

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125-PD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

125-PD

Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 50 µg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF

PDGF was originally discovered as a major mitogenic factor in serum but not in plasma. PDGF is stored in platelet alpha  granules and released upon platelet activation. Besides megakaryocytes, other cell types, including endothelial cells, monocyte/macrophages, vascular smooth muscle cells, fibroblasts, cytotrophoblasts and a variety of transformed or neoplastic cells, have been shown to produce PDGF. PDGFs are disulfide-linked dimers. The subunits of the PDGF dimers are homologous polypeptides designated PDGF-A and PDGF-B chains. Natural PDGFs can exist either as homodimers (PDGF-AA, PDGF-BB) or heterodimers (PDGF-AB). Although all three isoforms of PDGF exist in human platelets, R&D Systems’ hPDGF consists predominantly of hPDGF-AB heterodimers. PDGF obtained from porcine platelets contain only PDGF-BB homodimers.

Two distinct PDGF receptors, the alpha -receptor and the beta -receptor, have been identified. The two receptors are structurally related, with an extracellular portion containing five immunoglobulin-like domains, a single transmembrane region, and an intracellular portion with a protein-tyrosine kinase domain. The alpha -receptor binds both the A and B chains with high affinity whereas the beta -receptor binds only the B-chain with high affinity. Receptor dimerization is induced upon ligand binding.

In addition to being a potent mitogen for cells of mesenchymal origin, PDGF has also been shown to be a potent chemoattractant for mesenchymal cells, mononuclear cells and neutrophils and has been reported to be important in the modification of cellular matrix constituents.

Long Name
Platelet-derived Growth Factor
Entrez Gene IDs
5154 (Human)
Alternate Names
PDGF; PDGF1; PDGF-1; PDGFA; PDGF-A

Citations for Porcine PDGF Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Transcription factor scleraxis vitally contributes to progenitor lineage direction in wound healing of adult tendon in mice
    Authors: T Sakabe, K Sakai, T Maeda, A Sunaga, N Furuta, R Schweitzer, T Sasaki, T Sakai
    J. Biol. Chem., 2018-03-05;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Thymosin beta4 increases the potency of transplanted mesenchymal stem cells for myocardial repair.
    Authors: Ye L, Zhang P, Duval S, Su L, Xiong Q, Zhang J
    Circulation, 2013-09-10;128(11):S32-41.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Expression of S100A6 in cardiac myocytes limits apoptosis induced by tumor necrosis factor-alpha.
    Authors: Tsoporis JN, Izhar S, Parker TG
    J. Biol. Chem., 2008-08-27;283(44):30174-83.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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