Home / Buffers, Chelators and Reagents / preQ1-biotin

preQ1-biotin

Catalog #: 7804 Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
7804/5
preQ1-biotin | CAS No. 2971850-25-4 | Buffers, Chelators and Reagents
3 Images
Description: PreQ1 with biotin handle for RNA labeling and purification

Chemical Name: N-(6-(((2-Amino-4-oxo-4,7-dihydro-3H-pyrrolo[2,3-d]pyrimidin-5-yl)methyl)amino)hexyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide

Purity: ≥95%

Product Details
Reviews
Biological Activity Scientific Data Technical Data Additional Information Product Datasheets Calculators

Biological Activity

preQ1-biotin is a modified analog of the natural substrate prequeosine1 (preQ1) and is used for RNA-TAG (transglycosylation at guanosine) and DNA-TAG. preQ1-biotin enables affinity tagging and pull-down of specific RNAs that have been modified selectively by E. coli tRNA guanine transglycosylase (TGT). preQ1-biotin is incorporated site-specifically and covalently into RNAs containing a short harpin nucleotide recognition motif. The guanine in a UGU recognition element is exchanged with the preQ1-biotin substrate.

Plasmids for expression of the E.coli TGT enzyme (#138201) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end (#138209) are available from Addgene.

Scientific Data

Structure Schematic representation of preQ1-bitoin insertion into DNA. View Larger

E. coli TGT irreversibly exchanges a specific guanine in a DNA hairpin with preQ1-biotin. Schematic representation of preQ1-bitoin insertion into DNA.

Data Urea-PAGE gel shift analysis of preQ1-biotin insertion into a DNA View Larger

E. coli TGT irreversibly exchanges a specific guanine in a DNA hairpin with preQ1-biotin. Urea-PAGE gel shift analysis of preQ1-biotin insertion. Modification is evidenced by an upward shift of the product band due to molecular weight increase. Lane 1 is unmodified DNA; lane 2 is preQ1-biotin modified DNA.

Technical Data

M.Wt:
504.65
Formula:
C23H36N8O3S
Solubility:
Soluble to 20 mM in DMSO and to 5 mM in ethanol
Purity:
≥95%
Storage:
Store at -20°C
CAS No:
2971850-25-4

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

Licensing Caveats:
Sold under license from The Regents of the University of California.

Product Datasheets

Reconstitution Calculator
Molarity Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Molarity Calculator

=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

FAQs

No product specific FAQs exist for this product, however you may

View all Small Molecule FAQs

Reviews for preQ1-biotin

There are currently no reviews for this product. Be the first to review preQ1-biotin and earn rewards!

Have you used preQ1-biotin?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review
Tocris Bioscience is the leading supplier of novel and exclusive tools for life science research with over 30 years' experience in the industry. Tocris is a Bio-Techne brand.