Primate IFN-gamma ELISpot Kit

Catalog #: EL961
4 Citations Datasheet / COA / SDS

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EL961

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Summary Product Datasheets Preparation and Storage Background

Primate IFN-gamma ELISpot Kit Summary

Assay Type

Quantitative Sandwich ELISA

Format

96-well PVDF-backed microplate

Assay Length

3 hours 15 mins to 4 hours 30 mins*

Sample Type

Whole Cells

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

PRODUCT SUMMARY
ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. This kit is designed for the detection and enumeration of primate IFN-gamma. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

This ELISpot assay employs a capture antibody specific for primate IFN-gamma, pre-coated onto a PVDF-backed microplate. Appropriately stimulated cells are pipetted directly into the wells and the immobilized antibody in the immediate vicinity of the secreting cells binds secreted primate IFN-gamma. Following wash steps and incubation with a biotinylated detection antibody, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washing and a substrate solution (BCIP/NBT) is added. A blue-black colored precipitate forms at the sites of cytokine localization and appears as spots, with each individual spot representing an individual primate IFN-gamma secreting cell. The spots can be counted with an automated ELISpot reader system or manually using a stereomicroscope.

PRODUCT FEATURES

Detect and quantitate individual cells secreting primate IFN-gamma
High sensitivity - ELISpot assays can measure responses with frequencies well below 1 in 100,000 cells
No in vitro expansion of cells required
High-throughput - ELISpot assays use only a small number of primary cells

KIT CONTENTS

Primate IFN-gamma Microplate
Biotinylated Detection Antibody
Streptavidin conjugated to Alkaline Phosphatase
Dilution Buffers
Wash Buffer Concentrate
BCIP/NBT Chromogen
Primate IFN-gamma Positive Control

OTHER REAGENTS REQUIRED

Pipettes and pipette tips
Deionized or distilled water
Squirt bottle, manifold dispenser, or automated microplate washer
500 mL graduated cylinder
37 °C CO₂ incubator
Sterile culture media
Dissection microscope or an automated ELISpot reader

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name:

Interferon gamma

Entrez Gene IDs:

3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)

Alternate Names:

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for Primate IFN-gamma ELISpot Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Protective effects of the Francisella tularensis Î´pdpC mutant against its virulent parental strain SCHU P9 in Cynomolgus macaques
Authors: D Tian, A Uda, Y Ami, A Hotta, ES Park, N Nagata, N Iwata-Yosh, A Yamada, K Hirayama, K Miura, Y Koyama, M Azaki, S Morikawa
Sci Rep, 2019-06-24;9(1):9193.
Species: Rhesus Macaca (Macaque)
Sample Types: Whole Cells
IL-7 receptor blockade blunts antigen-specific memory T cell responses and chronic inflammation in primates
Authors: L Belarif, C Mary, L Jacquemont, HL Mai, R Danger, J Hervouet, D Minault, V Thepenier, V Nerrière-D, E Nguyen, S Pengam, E Largy, A Delobel, B Martinet, S Le Bas-Ber, S Brouard, JP Soulillou, N Degauque, G Blancho, B Vanhove, N Poirier
Nat Commun, 2018-10-26;9(1):4483.
Species: Primate
Sample Types: Whole Cells
Gene therapy for rhesus monkeys heterozygous for LDL receptor deficiency by balloon catheter hepatic delivery of helper-dependent adenoviral vector.
Authors: Oka K, Mullins C, Kushwaha R, Leen A, Chan L
Gene Ther, 2014-09-18;22(1):87-95.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Rhesus macaques vaccinated with consensus envelopes elicit partially protective immune responses against SHIV SF162p4 challenge.
Authors: Eugene H, Pierce-Paul B, Cragio J, Ross T
Virol J, 2013-04-02;10(0):102.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells