ProDots Recombinant Human IL-15 GMP Protein

Name: ProDots Recombinant Human IL-15 GMP Protein
Brand: R&D Systems
SKU: PRD247-GMP

Catalog #: PRD247-GMP Datasheet / COA / SDS

5 µg of GMP rhIL-15 supplied as a protein ball in a bag

Discontinued Product

PRD247-GMP has been discontinued.

View all IL-15 products.

5 μg of GMP Human IL-15 as one spheroid of 5 μg inside a sterile bag with a needless valve port and weldable tube port.

3 Images

All IL-15 Proteins and Enzymes

Product Details

FAQs

Reviews

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

ProDots Recombinant Human IL-15 GMP Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is 0.3-2.6 ng/mL.

The specific activity of ProDots Recombinant Human IL-15 GMP is >2.0 x 10⁸ units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554).

Source

E. coli-derived
Produced using non-animal reagents in an animal-free laboratory.
Manufactured and tested under cGMP guidelines.

Accession #

P40933.1

N-terminal Sequence
Analysis

Asn-Trp-Val-Asn-Val-Ile-Ser-Asp-Leu-Lys

Predicted Molecular Mass

12.5 kDa

Product Datasheets

PRD247-GMP

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

PRD247-GMP

Formulation	Lyophilized from an phosphate buffered saline-based formulation using proprietary excipients.
Reconstitution	Reconstitute immediately prior to use with up to 25 mL of cell culture media.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Store material as supplied, unopened, and unreconstituted at 2-8 °C until use. Stability is a minimum of 8 weeks when stored at 2-8 °C as supplied. Refer to lot specific COA for the Use by Date. The foil pouch is used for shipping and storage only and is not terminally sterilized. GMP ProDots within the weldable bag are prepared under GMP-controlled conditions and protein content is tested to USP <71> guidelines.

Scientific Data

Binding Activity

View Larger

GMP-grade ProDots^®Recombinant Human IL-15 (Catalog # PRD247-GMP) stimulates cell proliferation of MO7e human megakaryocytic leukemic cells. Three independent lots were tested for activity and plotted on the same graph to show lot-to-lot consistency of GMP ProDots IL-15.

N/A

View Larger

Comparative tests for function within biologically relevant model systems show equivalence of GMP ProDot™ Proteins and GMP Proteins. Activation of PBMCs after 9 days in culture with ExCellerate Human T Cell Media CCM030, GMP Cloudz Human T Cell Activation Kit CLD001-GMP and GMP IL-15 ProDot PRD247-GMP or GMP IL-15 247-GMP shows comparable A. T cell fold expansion and B. phenotypes

Reconstitution Calculator

Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). IL-15 binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (7). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8⁺ memory cells (12 - 16). An important component of these functions is the ability of
IL-15 to induce dendritic cell differentiation and inflammatory activation (11, 14). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8⁺ T cells (17). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (18).

References

De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
Grabstein, K. et al. (1994) Science 264:965.
Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
Giri, J.G. et al. (1995) EMBO J. 14:3654.
Giri, J. et al. (1994) EMBO J. 13:2822.
Dubois, S. et al. (2002) Immunity 17:537.
Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
Mortier, E. et al. (2004) J. Immunol. 173:1681.
Bergamaschi, C. et al. (2012) Blood 120:e1.
Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
Mortier, E. et al. (2003) J. Exp. Med. 205:1213.
Gordy, L.E. et al. (2011) J. Immunol. 187:6335.
Harris, K.M. (2011) J. Leukoc. Biol. 90:727.
Xia, J. et al. (2010) Clin. Immunol. 134:130.
Schluns, K.S. et al. (2002) J. Immunol. 168:4827.
Davies, E. et al. (2010) J. Leukoc. Biol. 88:529.
Barra, N.G. et al. (2010) Obesity 18:1601.

Long Name

Interleukin 15

Entrez Gene IDs

3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)

Alternate Names

IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

Product Specific Notices

The GMP ProDot bag(s) should be used after proper inspection. Assess the bags after media is added to the bag either through the weldable port or needless valve. Inspect the bag to ensure complete dissolution of the GMP ProDot, and visually inspect seals and corners for a complete seal.

GMP ProDots are fragile. Please handle with care. If breakage of a ProDot is observed, there is no integrity lost, and can be used as indicated.

SDS-PAGE Purity, Host Cell DNA, and Host Cell Protein testing were completed on protein prior to lyophilization.

The End User is aware that R&D Systems, Inc. sells GMP products for preclinical or clinical ex vivo use and not for in vivo use. The End User Terms of Use of Product may be found at: RnDSystems.com/legal-information.