Protein A Antibody

Catalog #: MAB99761 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB99761-100
MAB99761-SP

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All Protein A Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Protein A Antibody Summary

Specificity

Detects recombinant Protein A in direct ELISA and Western blots.

Source

Monoclonal Rat IgG_2A Clone # 946418

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Recombinant Protein A

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

20 µg/mL

Recombinant Protein A Long and Recombinant Protein A

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Protein A by Western Blot. Western blot shows Recombinant Protein A Long, Recombinant Protein A, and Recombinant Protein G (negative control). The left panel of the PVDF membrane was probed with 2 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99761) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Protein A at approximately 33 kDa and Protein A Long at approximately 55 kDa (as indicated). To show specificity, the right panel was probed with Anti-Rat IgG Antibody (negative control). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Protein A by Simple Western^TM. Simple Western lane view recombinant Protein A Long, recombinant Protein A, and recombinant Protein G (negative control), loaded at 0.2 mg/mL. A specific band was detected for Protein A at approximately 40 kDa and Protein A Long at approximately 58 kDa (as indicated) using 20 µg/mL of Rat Anti-Protein A Monoclonal Antibody (Catalog # MAB99761) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protein A

The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is used in purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.

Entrez Gene IDs

3919448 (S. aureus)

Alternate Names

Immunoglobulin G binding protein A; PROA; Protein A; Protein-A; SPA; Staphylococcal protein A