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Purmorphamine

Catalog #: 4551
22 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
4551/10
4551/50
Purmorphamine | CAS No. 483367-10-8 | Smoothened Receptor Agonists
2 Images
Description: Smo receptor agonist

Chemical Name: 9-Cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine

Purity: ≥98%

Product Details
Citations (22)
Supplemental Products
Reviews
Biological Activity Scientific Data Technical Data Background Product Datasheets Calculators

Biological Activity

Purmorphamine is a smoothened (Smo) receptor agonist (EC50 ~ 1 μM) and activates the Hedgehog pathway. Purmorphamine induces osteogenesis in mouse mesenchymal progenitor cells (C3H10T1/2). When combined with BMP-4, Purmorphamine can transdifferentiate pre-adipocytes (3T3-L1) and myoblasts (C2C12) into osteoblasts. Purmorphamine induces differentiation of multipotent mesenchymal progenitor cells into osteoblasts, and of spinal motor neurons from pluripotent human stem cells, and protects dopaminergic neurons in a mouse model of Parkinson's disease.

Scientific Data

N/A applications of purmorphamine in neurons derived from hiPSCs View Larger

Applications of Purmorphamine in Neurons Derived from hiPSCs Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT (Catalog # 2634, Tocris) and Purmorphamine. Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.

Technical Data

M.Wt:
520.62
Formula:
C31H32N6O2
Solubility:
Soluble to 100 mM in DMSO
Purity:
≥98%
Storage:
Store at -20°C
CAS No:
483367-10-8

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

  1. Single-Cell Sequencing of iPSC-Dopamine Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes
    C Lang, KR Campbell, BJ Ryan, P Carling, M Attar, J Vowles, OV Perestenko, R Bowden, F Baig, M Kasten, MT Hu, SA Cowley, C Webber, R Wade-Marti
    Cell Stem Cell, 2018;0(0):.
  2. Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway.
    Wu et al.
    Chem.Biol., 2004;11:1229
  3. Purmorphamine activates the Hedgehog pathway by targeting Smoothened.
    Sinha et al.
    Nat.Chem.Biol., 2006;2:29
  4. A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells.
    Wu et al.
    J.Am.Chem.Soc., 2002;124:14520

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Citations for Purmorphamine

The citations listed below are publications that use Tocris products. Selected citations for Purmorphamine include:

22 Citations: Showing 1 - 10

  1. Generation and characterization of NGLY1 patient-derived midbrain organoids.
    Authors: Wei Et al.
    Front Cell Dev Biol  2023;11:1039182
  2. Dynamic 3D Combinatorial Generation of hPSC-Derived Neuromesodermal Organoids With Diverse Regional and Cellular Identities.
    Authors: Mustafa Et al.
    Curr Protoc  2022;2:e568
  3. Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems.
    Authors: Lu Et al.
    Nat Protoc  2022;17:2699-2719
  4. Primary cilia and SHH signaling impairments in human and mouse models of Parkinson's disease.
    Authors: Beate Et al.
    Nat Commun  2022;13:4819
  5. Genome-wide identification of the genetic basis of amyotrophic lateral sclerosis.
    Authors: Cheng Et al.
    Neuron  2022;110:992-1008.e11
  6. ARP-T1-associated Bazex-Dupré-Christol syndrome is an inherited basal cell cancer with ciliary defects characteristic of ciliopathies.
    Authors: Fanny Et al.
    Commun Biol  2021;4:544
  7. Growth factor mimetics for skin regeneration: In vitro profiling of primary human fibroblasts and keratinocytes.
    Authors: Willeke F Et al.
    Arch Pharm (Weinheim)  2021;354:e2100082
  8. Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules.
    Authors: Ruili Et al.
    Methods Mol Biol  2021;2454:811-827
  9. Robust single-cell discovery of RNA targets of RNA-binding proteins and ribosomes.
    Authors: Jason G Et al.
    Nat Methods  2021;18:507-519
  10. Variable Outcomes in Neural Differentiation of Human PSCs Arise from Intrinsic Differences in Developmental Signaling Pathways.
    Authors: Frederick J Et al.
    Cell Rep  2020;31:107732
  11. Transcriptomic and epigenomic dynamics associated with development of human iPSC-derived GABAergic interneurons.
    Authors: Ying Et al.
    Hum Mol Genet  2020;29:2579-2595
  12. IPSC-Derived Neuronal Cultures Carrying the Alzheimer's Disease Associated TREM2 R47H Variant Enables the Construction of an Aβ-Induced Gene Regulatory Network.
    Authors: Kristel Et al.
    Int J Mol Sci  2020;21
  13. Single-Cell Sequencing of iPSC-DA Neurons Reconstructs Disease Progression and Identifies HDAC4 as a Regulator of Parkinson Cell Phenotypes.
    Authors: Lang Et al.
    Cell Stem Cell  2019;24:93
  14. Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling.
    Authors: Georg C Et al.
    Methods Mol Biol  2019;1994:31-39
  15. FGF Modulates the Axial Identity of Trunk hPSC-Derived Neural Crest but Not the Cranial-Trunk Decision.
    Authors: Hackland Et al.
    Stem Cell Reports  2019;12:920
  16. IFT80 is required for stem cell proliferation, differentiation, and odontoblast polarization during tooth development.
    Authors: Yuan Et al.
    Cell Death Dis  2019;10:63
  17. A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation.
    Authors: Sheng Et al.
    Nat Commun  2018;9:4047
  18. Th17 lymphocytes induce neuronal cell death in a human iPSC-based model of Parkinson's disease.
    Authors: Sommer Et al.
    Cell Stem Cell.  2018;23:123
  19. Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone Responses.
    Authors: Rajamani Et al.
    Cell Stem Cell  2018;22:698
  20. Zooming in on Cryopreservation of hiPSCs and Neural Derivatives: A Dual-Center Study Using Adherent Vitrification.
    Authors: Kaindl Et al.
    Stem Cells Transl Med  2018;
  21. Ciliary IFT80 balances canonical versus non-canonical hedgehog signalling for osteoblast differentiation.
    Authors: Yuan Et al.
    J Immunol  2016;7:11024
  22. Characterization of axon formation in the embryonic stem cell-derived motoneuron.
    Authors: Shinn-Zong Et al.
    Cell Transplant  2010;20:493-502

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