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Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Catalog #: NL006
14 Citations Datasheet / COA / SDS
NorthernLights 493 Fluorochrome-labeled Antibody, X Absorbed
Catalog # Availability Size / Price Qty
NL006
Detection of Rabbit IgG Primary Antibody by Immunocytochemistry.
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Citations (14)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rabbit IgG NorthernLights™ NL493-conjugated Antibody Summary

Species Reactivity
Rabbit
Specificity
Rabbit IgG (H+L)
Source
Polyclonal Donkey IgG
Purification
Antigen Affinity-purified
Immunogen
Rabbit IgG
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.
Label
NL493 (Excitation= 493 nm, Emission= 514 nm)

Applications

Recommended Concentration
Sample
Immunocytochemistry
Use at a 1:200 dilution
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry View Larger

Detection of Rabbit IgG Primary Antibody by Immunocytochemistry. Detection of human Ki-67 by incubating cells with 3 μg/mL of Rabbit anti-Human Ki-67 monoclonal antibody (MAB7617) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G
Alternate Names
IgG; Immunoglobulin G; ImmunoglobulinG

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Citations for Rabbit IgG NorthernLights™ NL493-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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  1. Influence of glucose and oxygen tension on the trophectoderm and the inner cell mass of in vitro produced bovine embryos
    Authors: Berling, FP;Mendes, CM;Goissis, MD;
    Theriogenology
    Species: Bovine
    Sample Types: Embryo
    Applications: Immunohistochemistry
  2. Fibronectin, DHPS and SLC3A2 Signaling Cooperate to Control Tumor Spheroid Growth, Subcellular eIF5A1/2 Distribution and CDK4/6 Inhibitor Resistance
    Authors: C Geller, J Maddela, R Tuplano, F Runa, Y Adamian, R Güth, GO Soto, L Tomaneng, J Cantor, JA Kelber
    bioRxiv : the preprint server for biology, 2023-04-16;0(0):.
  3. Obesity defined molecular endotypes in the synovium of patients with osteoarthritis provides a rationale for therapeutic targeting of fibroblast subsets
    Authors: Susanne N. Wijesinghe, Amel Badoume, Dominika E. Nanus, Archana Sharma‐Oates, Hussein Farah, Michelangelo Certo et al.
    Clinical and Translational Medicine
  4. Spontaneous Osteoclastogenesis, a risk factor for bone metastasis in advanced luminal A-type breast cancer patients
    Authors: Valeria Fernández Vallone, Francisco Raúl Borzone, Leandro Marcelo Martinez, María Belén Giorello, Hosoon Choi, Federico Dimase et al.
    Frontiers in Oncology
  5. CD200S-positive granulated lymphoid cells in endometrium appear to be CD56-positive uterine NK cells
    Authors: DA Clark, A Reihani, JL Arredondo, K Ask, WG Foster
    Journal of reproductive immunology, 2022-01-14;150(0):103477.
    Species: Human
    Sample Types: Whole Tissues
    Applications: IHC
  6. Further Characterization of Rio Grande Virus and Potential for Cross Reactivity with Rift Valley Fever Virus Assays
    Authors: MS Szymczak, WK Reeves, MM Miller
    Viruses, 2021-08-30;13(9):.
    Species: Cercopithecus aethiops
    Sample Types: Whole Cells
    Applications: ICC
  7. Telomerase reverse transcriptase ameliorates lung fibrosis by protecting alveolar epithelial cells against senescence
    Authors: T Liu, F Gonzalez D, Y Zhao, Z Wu, AE Rinke, KK Kim, SH Phan
    J. Biol. Chem., 2019-04-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Neuroprotective role of kolaviron in striatal redo-inflammation associated with rotenone model of Parkinson's disease
    Authors: EO Farombi, IO Awogbindin, TH Farombi, JO Oladele, ER Izomoh, OB Aladelokun, IO Ezekiel, OI Adebambo, VO Abah
    Neurotoxicology, 2019-03-28;73(0):132-141.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. Endothelial autophagy and Endothelial-to-Mesenchymal Transition (EndoMT) in eEPC treatment of ischemic AKI
    Authors: Daniel Patschan, Katrin Schwarze, Elvira Henze, Susann Patschan, Gerhard Anton Müller
    Journal of Nephrology
  10. The Dual NOD1/NOD2 Agonism of Muropeptides Containing a Meso-Diaminopimelic Acid Residue
    PLoS ONE, 2016-08-11;11(8):e0160784.
    Applications: Flow Cytometry
  11. Tiam1-Rac1 Axis Promotes Activation of p38 MAP Kinase in the Development of Diabetic Retinopathy: Evidence for a Requisite Role for Protein Palmitoylation
    Authors: Rajakrishnan Veluthakal, Binit Kumar, Ghulam Mohammad, Anjaneyulu Kowluru, Renu A. Kowluru
    Cellular Physiology and Biochemistry
  12. Primitive stem cells derived from bone marrow express glial and neuronal markers and support revascularization in injured retina exposed to ischemic and mechanical damage.
    Stem Cells Dev., 2011-10-24;21(9):1488-500.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  13. A DNMT3B Alternatively Spliced Exon and Encoded Peptide Are Novel Biomarkers of Human Pluripotent Stem Cells.
    Authors: Gopalakrishna-Pillai S, Iverson LE
    PLoS ONE, 2011-06-15;6(6):e20663.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  14. Tumor necrosis factor receptor expression and signaling in renal cell carcinoma.
    Authors: Al-Lamki RS, Sadler TJ, Wang J
    Am. J. Pathol., 2010-06-21;177(2):943-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

  1. Are products derived from donkey serum listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)?
    • All products derived from donkeys were produced from the sera of domestic donkeys of a genus and species not protected by CITES. No CITES permit is required for the transportation of these materials.
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