Rabbit VisUCyte HRP Polymer-DAB Cell & Tissue Staining Kit

Detection of Ki67/MKI67 in Kidney Tumor. Tissue sections were first incubated with Human Ki67/MKI67 Antibody (MAB7617) and then subjected to staining development using Rabbit IgG VisUCyte HRP-DAB Kit (Catalog # VCTS003B). Specific staining was localized to the nucleus. Note strong and high contrast specific tissue staining and the absence of non-specific background staining produced by the VisUCyte kit.

Detection of ErbB2/Her2 in human breast cancer Tissue sections were first incubated with Rabbit Anti-Human Phospho-ErbB2/Her2 Polyclonal Antibody (AF1768) and then subjected to staining development using Rabbit VisUCyte Detection kit (Catalog# VCTS003B). Staining is confined to the cell membranes of cancer cells. Note strong and high contrast specific tissue staining and the absence of non-specific background staining produced by the VisUCyte kit.

Side-by-side comparison of staining using different detection chemistries on adjacent tissue sections of Human Colon Tumor. Ki67/MKI67 was detected in immersion fixed paraffin-embedded sections of human colon tumor using Rabbit Anti-Human Ki67/MKI67 Monoclonal Antibody (Catalog # MAB7617) at 1 µg/ml for overnight at 4 °C followed by incubation with the Rabbit VisUCyte HRP Polymer-DAB Cell & Tissue Staining Kit (Catalog # VCTS003B) and a generic Avidin-Biotin kit. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. Strong specific tissue staining is seen using VisUCyte kit. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.