Rat Adiponectin/Acrp30 Antibody

Catalog #: AF3100 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3100
AF3100-SP

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Rat Adiponectin/Acrp30 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross-reactivity with recombinant mouse Adiponectin is observed and less than 10% cross-reactivity with recombinant human Adiponectin is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Adiponectin/Acrp30
Ile19-Asn244
Accession # NP_653345

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Rat Adiponectin/Acrp30

Immunohistochemistry

5-15 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Adiponectin/Acrp30 in Rat Liver. Adiponectin/Acrp30 was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Rat Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3100) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry

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Adiponetin/Acrp30 in Rat Adipocytes. Adiponectin/Adipocyte complement-related protein of 30 kDa (ACRP-30) was detected in rat adipocytes using Goat Anti-Rat Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

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Adiponectin/Acrp30 in Mouse Adipocytes. Adiponectin/Acrp30 was detected in immersion fixed mouse mesenchymal stem cells differentiated into adipocytes using Goat Anti-Rat Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Adiponectin/Acrp30

Adiponectin, also known as Adipocyte complement-related protein of 30 kDa (Acrp30), is a secreted adipose tissue-specific protein. It is an anti-atherogenic, anti-diabetic and anti-inflammatory hormone that plays important roles in the regulation of lipid and glucose metabolism. Rat Adiponectin/Acrp30 shares 91% and 83% amino acid sequence homology with mouse and human Adiponectin/Acrp30, respectively.

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Alternate Names

ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein