Rat CCL3/MIP-1 alpha Antibody

Catalog #: MAB66251 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB66251
MAB66251-SP

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Rat CCL3/MIP-1 alpha Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CCL3/MIP-1 alpha in direct ELISAs. In direct ELISAs, less than 50% cross-reactivity with recombinant mouse and cotton rat CCL3/MIP-1 alpha is observed, and no cross-reactivity with recombinant human, canine, and HHV CCL3/MIP-1 alpha, recombinant rat CCL4/MIP-1 beta, or recombinant mouse CCL5/RANTES is observed.

Source

Monoclonal Mouse IgG_2B Clone # 769303

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rat CCL3/MIP-1 alpha
Ala24-Ala92
Accession # P50229

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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CCL3/MIP‑1 alpha in Rat Kidney. CCL3/MIP-1a was detected in perfusion fixed frozen sections of rat kidney using Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66251) at 25 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of epithelial cells in convoluted tubules. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature rat CCL3 shares 74%, 91%, and 88% amino acid sequence identity with human, mouse, and cotton rat CCL3, respectively (2). CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (3, 4). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (5, 6). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (6). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (7). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 8-10). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (3). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (11-14), although it also can suppress the replication of HIV (15). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1-mediated noxious stimulation (16).

References

Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Shi, M.M. et al. (1995) Biochem. Biophys. Res. Commun. 211:289.
Graham, G.J. et al. (1990) Nature 344:442.
Wagner, L. et al. (1998) Nature 391:908.
Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
Ren, M. et al. (2010) EMBO J. 29:3952.
Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
Taub, D.D. et al. (1993) Science 260:355.
Bernardini, G. et al. (2008) Blood 111:3626.
Lee, S.C. et al. (2000) J. Immunol. 164:3392.
Wu, Y. et al. (2008) J. Immunol. 181:6384.
Cook, D.N. et al. (1995) Science 269:1583.
Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
Cocchi, F. et al. (1995) Science 270:1811.
Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.

Entrez Gene IDs

6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)

Alternate Names

C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta