Rat CCL3/MIP-1 alpha Antibody

Catalog #: MAB66252
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB66252
MAB66252-SP

Detection of Rat CCL3/MIP‑1 alpha by Western Blot.

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CCL3/MIP-1 alpha Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CCL3/MIP‑1 alpha in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 769324

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rat CCL3/MIP‑1 alpha
Ala24-Ala92
Accession # P50229

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Neutralization

Measured by its ability to neutralize CCL3/MIP‑1 alpha -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR5. The Neutralization Dose (ND₅₀) is typically 0.075-0.45 μg/mL in the presence of 10 ng/mL Recombinant Rat CCL3/MIP‑1 alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat CCL3/MIP‑1 alpha by Western Blot. Western blot shows lysates of NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66252) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL3/MIP-1a at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization

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Chemotaxis Induced by CCL3/MIP‑1 alpha and Neutral-ization by Rat CCL3/ MIP‑1 alpha Antibody. Recombinant Rat CCL3/MIP-1a (Catalog # 6625-MA) induces chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Rat CCL3/MIP-1a (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat CCL3/MIP-1a Monoclonal Antibody (Catalog # MAB66252). The ND₅₀ is typically 0.075-0.45 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL3/MIP-1 alpha

CCL3, also known as macrophage inflammatory protein 1 alpha (MIP-1 alpha ) and LD78, is a member of the beta or CC subfamily of chemokines and is closely related to CCL4/MIP-1 beta. Chemokines comprise a large family of small secreted proteins that are involved in immune and inflammatory responses. CCL3 expression can be induced in a variety of hematopoietic cells, fibroblasts, smooth muscle cells, and epithelial cells (1). Mature rat CCL3 shares 74%, 91%, and 88% amino acid sequence identity with human, mouse, and cotton rat CCL3, respectively (2). CCL3 is an approximately 8 kDa chemokine that forms complexes with sulfated proteoglycans (3, 4). In a reversible process, CCL3 associates into noncovalently-linked dimers which then form tetramers and high molecular weight polymers (5, 6). These complexes of CCL3 are protected from proteolytic digestion by insulin degrading enzyme (IDE) which can cleave the monomeric chemokine (6). CCL3 exerts its biological functions through interactions with CCR1, CCR3, and CCR5 (1). It is cleared from the extracellular space by internalization via the decoy chemokine receptor D6 (7). CCL3 promotes the chemoattraction, adhesion to activated vascular endothelium, and cellular activation of many hematopoietic cell types including activated T cells, NK cells, neutrophils, monocytes, immature dendritic cells, and eosinophils (1, 8-10). CCL3 is also known as stem cell inhibitor (SCI) and can inhibit the proliferation of hematopoietic progenitor cells (3). CCL3 bioactivity contributes to tumor metastasis and the inflammatory components of viral infection, rheumatoid arthritis, and hepatitis (11-14), although it also can suppress the replication of HIV (15). CCL3 additionally promotes hyperalgesia by sensitizing sensory neurons to TRPV1-mediated noxious stimulation (16).

References

Menten, P. et al. (2002) Cytokine Growth Factor Rev. 13:455.
Shi, M.M. et al. (1995) Biochem. Biophys. Res. Commun. 211:289.
Graham, G.J. et al. (1990) Nature 344:442.
Wagner, L. et al. (1998) Nature 391:908.
Graham, G.J. et al. (1994) J. Biol. Chem. 269:4974.
Ren, M. et al. (2010) EMBO J. 29:3952.
Weber, M. et al. (2004) Mol. Biol. Cell 15:2492
Taub, D.D. et al. (1993) Science 260:355.
Bernardini, G. et al. (2008) Blood 111:3626.
Lee, S.C. et al. (2000) J. Immunol. 164:3392.
Wu, Y. et al. (2008) J. Immunol. 181:6384.
Cook, D.N. et al. (1995) Science 269:1583.
Chintalacharuvu, S.R. et al. (2005) Immunol. Lett. 100:202.
Ajuebor, M.N. et al. (2004) Eur. J. Immunol. 34:2907.
Cocchi, F. et al. (1995) Science 270:1811.
Zhang, N. et al. (2005) Proc. Natl. Acad. Sci. 102:4536.

Entrez Gene IDs

6348 (Human); 20302 (Mouse); 25542 (Rat); 448787 (Canine); 102136134 (Cynomolgus Monkey)

Alternate Names

C-C motif chemokine 3; MIP1-(a); AI323804; CCL3; chemokine (C-C motif) ligand 3; G0S19-1; LD78a; LD78alpha; MIP1 alpha; MIP-1 alpha; MIP1A; MIP-1alpha; MIP1-alpha; PAT 464.1; SCYA3; SIS-beta

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Citations for Rat CCL3/MIP-1 alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Sublytic C5b-9 Induces CCL3/4 Production and Macrophage Accumulation in Thy-1N Rats via PKC-alpha /p65/IRF-8 Axis
Authors: Wenbo Wang, Baomei Qian, Chenhui Zhao, Mingyu Peng, Longfei Liu, Mengxiao Xie et al.
International Journal of Biological Sciences
A2BAR Antagonism Decreases the Glomerular Expression and Secretion of Chemoattractants for Monocytes and the Pro-Fibrotic M2 Macrophages Polarization during Diabetic Nephropathy
Authors: Torres-Arévalo, Á;Nahuelpán, Y;Muñoz, K;Jara, C;Cappelli, C;Taracha-Wiśniewska, A;Quezada-Monrás, C;Martín, RS;
International journal of molecular sciences
Species: Rat
Sample Types: Whole Cells, Whole Tissue
Applications: Immunohistochemistry, Neutralization
Involvement of the chemokine CCL3 and the purinoceptor P2X7 in the spinal cord in paclitaxel-induced mechanical allodynia.
Authors: Ochi-ishi, Ryutaro, Nagata, Kenichir, Inoue, Tomoyuki, Tozaki-Saitoh, Hidetosh, Tsuda, Makoto, Inoue, Kazuhide
Mol Pain, 2014-08-15;10(0):53.
Species: Rat
Sample Types: In Vivo
Applications: In Vivo