Rat CCR2 Antibody

Catalog #: MAB8368R Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB8368R-100
MAB8368R-SP

Detection of CCR2 in HEK293 Human Cell Line Transfected with Rat CCR2 and eGFP by Flow Cytometry

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat CCR2 Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CCR2 in direct ELISAs.

Source

Recombinant Monoclonal Mouse IgG_2B Clone # 890231R

Purification

Protein A or G purified from cell culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CCR2
Accession # O55193

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR2 in HEK293 Human Cell Line Transfected with Rat CCR2 and eGFP by Flow Cytometry HEK293 human embryonic kidney cell line transfected with either (A) rat CCR2 or (B) irrelevant protein and eGFP was stained with Mouse Anti-Rat CCR2 Monoclonal Antibody (Catalog # MAB8368R) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG2b isotype control antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Rat CCR2 shares 95% aa sequence identity with mouse CCR2.

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Alternate Names

C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor