Rat CCR2 PE-conjugated Antibody

Catalog #: FAB8368P
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB8368P-100UG

Detection of CCR2 in HEK293 Human Cell Line Transfected with Rat CCR2 and eGFP by Flow Cytometry.

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All CCR2 Antibodies

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Citations (1)

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Rat CCR2 PE-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Stains rat CCR2 transfectants but not irrelevant transfectants in flow cytometry.

Source

Monoclonal Mouse IgG_2B Clone # 890231

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with rat CCR2
Accession # O55193

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR2 in HEK293 Human Cell Line Transfected with Rat CCR2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with rat CCR2 and eGFP was stained with either (A) Mouse Anti-Rat CCR2 PE-conjugated Monoclonal Antibody (Catalog # FAB8368P) or (B) Mouse IgG_2BPhycoerythrin Isotype Control (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR2

CCR2, also known as CD192, is a 38 kDa 7TM chemokine receptor that preferentially binds CCL2, CCL7, and CCL13. CCR2 is expressed by multiple hematopoietic cells, endothelial cells, fibroblasts, neurons, and smooth muscle cells. It functions as an HIV fusion co-factor and facilitates T cell recruitment during inflammation. Two alternate splice forms (CCR2A and CCR2B) differ only by the addition of 14 amino acids to the intracellular carboxyl terminal. Rat CCR2 shares 95% aa sequence identity with mouse CCR2.

Entrez Gene IDs

729230 (Human); 12772 (Mouse); 60463 (Rat)

Alternate Names

C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CC-CKR-2CKR2B; CCR2; CCR-2; CCR2A; CCR2B; CD192 antigen; CD192; chemokine (C-C motif) receptor 2; CKR2; CKR2A; CMKBR2; CMKBR2MGC111760; FLJ78302; MCP-1 receptor; MCP-1-R; MCP-1-RMGC103828; MGC168006; Monocyte chemoattractant protein 1 receptor; monocyte chemotactic protein 1 receptor

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Citation for Rat CCR2 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Twist1 in podocytes ameliorates podocyte injury and proteinuria by limiting CCL2-dependent macrophage infiltration
Authors: J Ren, Y Xu, X Lu, L Wang, S Ide, G Hall, T Souma, JR Privratsky, RF Spurney, SD Crowley
Oncogene, 2021-08-09;6(15):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry