Rat CD161 Biotinylated Antibody

Catalog #: FAB9766B Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB9766B-100

Detection of Rat CD161 in Rat splenocytes by Flow Cytometry.

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Rat CD161 Biotinylated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat CD161 in ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 10/78

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Purified splenic NK cells from the LEW rat strain

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Biotin

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Rat CD161 in Rat splenocytes by Flow Cytometry. Rat splenocytes were stained with Anti-Rat CD3 APC-conjugated Monoclonal Antibody and either (A) Mouse Anti-Rat CD161 Biotinylated Monoclonal Antibody (Catalog # FAB9766B) or (B) Mouse IgG1 Biotinylated Isotype control (Catalog # IC002B)followed by Phycoerythrin-conjugated Streptavidin (Catalog # F0040). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD161

CD161, also known as KLRB1, NK1.1, and NKR-P1A is a 40 kDa type II transmembrane glycoprotein that contains one C-type lectin domain in its extracellular region. CD161 is expressed as a disulfide-linked dimer on the surface of Th17 cells and NK cells as well as on subsets of CD1-restricted T cells, intestinal NT cells, peripheral memory T cells, monocytes, and dendritic cells. It binds to OCIL/CLEC2d, leading to an inhibition of NK cell-mediated cytotoxicity and IFN-gamma secretion. Alternatively, CD161 can enhance TCR activation to CD1d ligation. CD161+ cell populations are depleted in ulcerative colitis, Grave's disease, and AIDS, although CD161+ T cells are activated during asthmatic attacks. Additional related proteins are expressed in mouse but not human: the inhibitory NKR-P1B and D, and the stimulatory NKR-P1A, C, and F.

Entrez Gene IDs

3820 (Human); 25192 (Rat)

Alternate Names

CD161 antigen; CD161; CLEC5B; CLEC5BC-type lectin domain family 5 member B; HNKR-P1a; killer cell lectin-like receptor subfamily B member 1; killer cell lectin-like receptor subfamily B, member 1; KLRB1; Ly59; Natural killer cell surface protein P1A; NKR; NKRP1; NKR-P1; NKRP1A; NKR-P1AMGC138614; NKRP1ANKR